The centrosome is an actin-organizing centre.
The centrosome is an actin-organizing centre.
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DOI：
10.1038/ncb3285
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发表时间：
2016-01
影响因子：
21.3
通讯作者：
Théry M
中科院分区：
文献类型：
--
作者：
Farina F;Gaillard J;Guérin C;Couté Y;Sillibourne J;Blanchoin L;Théry M
微管和肌动蛋白细丝是支持细胞内结构和细胞极性的两种主要细胞骨架网络。中心体核和锚定微管，因此被认为是主要的微管组织中心。然而，反复出现的但尚未解释的观察结果表明，中心体和肌动蛋白细丝之间存在联系。在这里，我们使用分离的中心体来证明中心体可以直接促进肌动蛋白细丝组装。在活细胞中也可以发现一团与中心体相关的肌动蛋白细丝。肌动蛋白-细丝在中心体的成核是由成核促进因子WASH和Arp2/3复合体共同介导的。中心三极周围物质1(PCM1)可能通过调节Arp2/3复合体来调节中心体肌动蛋白网络，并将其重新募集到中心体。因此，我们的结果揭示了中心体作为细胞内组织者的另一个方面，并为中心体如何作为肌动蛋白细丝组织中心发挥作用提供了机械性的见解。
Microtubules and actin filaments are the two main cytoskeleton networks supporting intracellular architecture and cell polarity. The centrosome nucleates and anchors microtubules and is therefore considered to be the main microtubule-organizing center. However, recurring, yet unexplained, observations have pointed towards a connection between the centrosome and actin filaments. Here we have used isolated centrosomes to demonstrate that the centrosome can directly promote actin filament assembly. A cloud of centrosome-associated actin filaments could be identified in living cells as well. Actin-filament nucleation at the centrosome was mediated by the nucleation promoting factor WASH in combination with the Arp2/3 complex. Pericentriolar material 1 (PCM1) appeared to modulate the centrosomal actin network by regulating Arp2/3 complex and WASH recruitment to the centrosome. Hence our results reveal an additional facet of the centrosome as an intracellular organizer and provide mechanistic insights into how the centrosome can function as an actin filament-organizing center.
DOI：
10.1083/jcb.86.1.87
发表时间：
1980-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kleve MG;Clark WH Jr
通讯作者：
Clark WH Jr