EFFECT OF SODIUM-SALICYLATE ON THE HUMAN HEAT-SHOCK RESPONSE
EFFECT OF SODIUM-SALICYLATE ON THE HUMAN HEAT-SHOCK RESPONSE
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DOI：
10.1126/science.1546322
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发表时间：
1992-03-06
期刊：
影响因子：
56.9
通讯作者：
MORIMOTO, RI
中科院分区：
文献类型：
--
作者：
JURIVICH, DA;SISTONEN, L;MORIMOTO, RI
水杨酸钠是一种抗炎剂，研究了其对培养的人类细胞热休克反应的影响。水杨酸激活的DNA结合的热休克转录因子（HSF）的热休克过程中获得的激活。然而，水杨酸钠并没有诱导热休克基因的转录，即使HSF在体内结合到热休克蛋白70（Hsp 70）基因上游的热休克元件。这些结果表明，热休克转录反应的激活是一个多步骤的过程。细胞外pH的调节增强了对水杨酸诱导的HSF激活的敏感性。
Sodium salicylate, an anti-inflammatory agent, was examined for its effects on the heat shock response in cultured human cells. Salicylate activation of DNA binding by the heat shock transcription factor (HSF) was comparable to activation attained during heat shock. However, sodium salicylate did not induce heat shock gene transcription even though the HSF was bound in vivo to the heat shock elements upstream of the heat shock protein 70 (Hsp 70) gene. These results reveal that activation of the heat shock transcriptional response is a multistep process. Modulation of extracellular pH augments sensitivity to salicylate-induced activation of HSF.