Quantitative trait loci analysis of flowering-time-related traits in tomato
Quantitative trait loci analysis of flowering-time-related traits in tomato
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DOI：
10.1016/j.scienta.2009.10.002
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发表时间：
2010-01
影响因子：
4.3
通讯作者：
M. R. Sumugat;O. Lee;K. Nemoto;N. Sugiyama
中科院分区：
文献类型：
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作者：
M. R. Sumugat;O. Lee;K. Nemoto;N. Sugiyama
开花时间的调节主要在光周期敏感的模式植物中进行研究。对光周期不敏感植物开花时间的环境和遗传调控知之甚少。本研究利用数量性状基因座（QTL）分析方法，对中性日番茄开花时间的遗传控制进行了研究。本研究以番茄（Solanum lycopersicum）‘M570018’及其野生近缘种茄（Solanum pimpinellifolium）为材料，构建了番茄BC 1F 6群体（PI 124039）来测量开花天数（DTF）和第一花序前的叶片数（LN），以及它们的潜在发育过程，包括叶片启动数（LI）、花诱导植株的百分比、开花诱导植株的数量和开花诱导植株的数量。（生殖指数，RI），肉眼可见的花芽出现的天数（DMB）和花发育持续时间（FDD：DTF-DMB）。复合区间作图检测到6个性状的12个QTL，其中2个QTL位于第1和第6染色体上。DTF QTL可解释43%的表型变异。S.检测到的QTLs中的pimpinellifolium等位基因提高叶片分化率，降低叶片层厚度，促进花的诱导、花的发育和开花。LN、LI、RI、DMB和FDD的QTL大多与DTF QTL聚在一起，dmb 1、fdd 1、li_14d1、li_19d1和ri_19d1与第1染色体上的dtf 1聚在一起，ln 6和fdd 6与第6染色体上的dtf 6聚在一起。这些结果表明，第1和第6染色体上的QTL可能形成一个功能性的“基因簇”，协同驱动番茄开花。另外，这两个DTF QTL可能作为“主基因”，通过对多个发育过程的多效性效应来控制开花时间。
The regulation of flowering time has been studied mainly in photoperiod-sensitive model plants. Little is known about the environmental and genetic regulation of flowering time in photoperiod-insensitive plants. Here, we studied the genetic control of flowering time in day-neutral tomato using quantitative trait locus (QTL) analysis. We used a BC1F6population developed from Solanum lycopersicum ‘M570018’ and its wild relative Solanum pimpinellifolium (PI124039) to measure days to flowering (DTF) and number of leaves preceding the first inflorescence (LN), as well as their underlying developmental processes, which include the number of leaves initiated (LI), percentage of flower-induced plants (reproductive index, RI), days to macroscopic flower bud appearance (DMB) and flower development duration (FDD: DTF−DMB). A composite interval mapping detected 12 QTLs for the six traits, which included two QTLs for DTF on chromosomes 1 and 6. The DTF QTLs explained 43% of the phenotypic variation of this trait. The presence of S. pimpinellifolium alleles in the detected QTLs increased the rate of leaf initiation, reduced LN and hastened flower induction, floral development and anthesis. Most QTLs for LN, LI, RI, DMB and FDD clustered with the DTF QTLs; dmb1, fdd1, li_14d1, li_19d1 and ri_19d1 clustered with dtf1 on chromosome 1, and ln6 and fdd6 clustered with dtf6 on chromosome 6. These results suggest that the QTLs on chromosomes 1 and 6 may form a functional “gene cluster” that drives tomato flowering in synergy. Alternatively, the two DTF QTLs may act as “master genes” that control flowering time through pleiotropic effects on multiple developmental processes.