Cultivable Gut Bacteria of Scarabs (Coleoptera: Scarabaeidae) Inhibit Bacillus thuringiensis Multiplication
Cultivable Gut Bacteria of Scarabs (Coleoptera: Scarabaeidae) Inhibit Bacillus thuringiensis Multiplication
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DOI：
10.1603/en14028
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发表时间：
2014-05
期刊：
影响因子：
2.7
通讯作者：
Yueming Shan;C. Shu;N. Crickmore;Chunqin Liu;W. Xiang;F. Song;Jie Zhang
中科院分区：
文献类型：
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作者：
Yueming Shan;C. Shu;N. Crickmore;Chunqin Liu;W. Xiang;F. Song;Jie Zhang
苏云金芽孢杆菌（Bacillus thuringiensis）是一种用于防治金龟子的昆虫病原菌，但其防治效果并不理想。肠道细菌对宿主具有多样的生态和进化效应，但肠道细菌是否能保护圣甲虫免受B的侵害。苏云金杆菌感染仍然知之甚少。为了研究这一点，我们从黑鳃金龟Faldermann，黑鳃金龟parallela Motschulsky，Anomala corpulenta Motschulsky分离出32种可培养的肠道细菌，并分析了它们对B的影响。苏云金杆菌增殖和Cry毒素稳定性。16S rDNA分析表明，这些肠道细菌属于变形菌门、放线菌门、厚壁菌门和拟杆菌门。32个分离物对3种金龟子特异性B的对峙培养分析。苏云金杆菌菌株的抗菌活性表明，大多数金龟子肠道细菌对B具有抗菌活性。苏云金杆菌菌株Cry毒素稳定性分析结果显示，虽然有几种菌株产生能够处理圣甲虫特异性毒素Cry8Ea的蛋白酶，但没有一种菌株能够完全降解它。这些结果表明，肠道细菌可能会影响圣甲虫对B的易感性。苏云金杆菌，在考虑今后的控制措施时应考虑到这一点。
ABSTRACT The entomopathogen Bacillus thuringiensis is used to control various pest species of scarab beetle but is not particularly effective. Gut bacteria have diverse ecological and evolutionary effects on their hosts, but whether gut bacteria can protect scarabs from B. thuringiensis infection remains poorly understood. To investigate this, we isolated 32 cultivable gut bacteria from Holotrichia oblita Faldermann, Holotrichia parallela Motschulsky, and Anomala corpulenta Motschulsky, and analyzed their effect on B. thuringiensis multiplication and Cry toxin stability. 16S rDNA analysis indicated that these gut bacteria belong to the Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes phyla. A confrontation culture analyses of the 32 isolates against three scarab-specific B. thuringiensis strains showed that the majority of the scarab gut bacteria had antibacterial activity against the B. thuringiensis strains. The Cry toxin stability analysis results showed that while several strains produced proteases capable of processing the scarab-specific toxin Cry8Ea, none were able to completely degrade it. These results suggest that gut bacteria can potentially affect the susceptibility of scarabs to B. thuringiensis and that this should be considered when considering future control measures.