Antibiotic selectivity for prokaryotic vs. eukaryotic decoding sites
Antibiotic selectivity for prokaryotic vs. eukaryotic decoding sites
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DOI：
10.1039/c0cc00423e
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发表时间：
2010-01-01
影响因子：
4.9
通讯作者：
Tor, Yitzhak
中科院分区：
文献类型：
--
作者：
Xie, Yun;Dix, Andrew V.;Tor, Yitzhak
FRET组装报告抗生素对两种不同RNA靶标的亲和力。用荧光团标记结合剂，所述荧光团充当细菌A位点上的发射性核苷的受体和末端标记的人A位点的供体荧光团。未标记的药物用于解离标记的抗生素。
A FRET assembly reports antibiotic affinities to two different RNA targets. A binder was labeled with a fluorophore that acts both as an acceptor for the emissive nucleoside on the bacterial A-site and a donor fluorophore for the terminally-labeled human A-site. Unlabeled drugs were used to dissociate the labeled antibiotic.