Matrix metalloproteinases are involved in mechanical stretch–induced activation of skeletal muscle satellite cells
Matrix metalloproteinases are involved in mechanical stretch–induced activation of skeletal muscle satellite cells
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DOI：
10.1002/mus.20601
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发表时间：
2006-09
期刊：
影响因子：
3.4
通讯作者：
M. Yamada;R. Tatsumi;T. Kikuiri;Shinpei Okamoto;Shinsuke Nonoshita;W. Mizunoya;Y. Ikeuchi;H. Shimokawa;K. Sunagawa;R. Allen
中科院分区：
文献类型：
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作者：
M. Yamada;R. Tatsumi;T. Kikuiri;Shinpei Okamoto;Shinsuke Nonoshita;W. Mizunoya;Y. Ikeuchi;H. Shimokawa;K. Sunagawa;R. Allen
当骨骼肌受到拉伸或损伤时，肌源性卫星细胞被激活进入细胞周期。这一过程取决于一氧化氮（NO）的产生，肝细胞生长因子（HGF）从细胞外基质的释放，以及HGF对c-met受体的呈递。本文报道的实验提供了新的证据，即基质金属蛋白酶（MMPs）参与体外HGF的NO依赖性释放。当大鼠卫星细胞用10 ng/ml MMPs的重组组织抑制剂-1（TIMP-1）处理并进行体外诱导活化的处理时，即，硝普钠（SNP）的NO供体或机械循环拉伸，激活反应被抑制。此外，由TIMP-1加SNP或机械拉伸处理的培养物产生的条件培养基不能激活培养的卫星细胞，并且不含HGF。此外，NOx测定表明，TIMP-1不损害伸展的卫星细胞培养物的NO合酶活性。因此，这些实验的结果提供了有力的证据，MMPs介导HGF从基质中释放，并且该途径中的这一步骤是NO合成的下游。肌肉神经，2006年
When skeletal muscle is stretched or injured, myogenic satellite cells are activated to enter the cell cycle. This process depends on nitric oxide (NO) production, release of hepatocyte growth factor (HGF) from the extracellular matrix, and presentation of HGF to the c‐met receptor. Experiments reported herein provide new evidence that matrix metalloproteinases (MMPs) are involved in the NO‐dependent release of HGF in vitro. When rat satellite cells were treated with 10 ng/ml recombinant tissue inhibitor‐1 of MMPs (TIMP‐1) and subjected to treatments that induce activation in vitro, i.e., sodium nitroprusside (SNP) of an NO donor or mechanical cyclic stretch, the activation response was inhibited. In addition, conditioned medium generated by cultures treated with TIMP‐1 plus SNP or mechanical stretch failed to activate cultured satellite cells and did not contain HGF. Moreover, NOx assay demonstrated that TIMP‐1 does not impair NO synthase activity of stretched satellite cell cultures. Therefore, results from these experiments provide strong evidence that MMPs mediate HGF release from the matrix and that this step in the pathway is downstream from NO synthesis. Muscle Nerve, 2006