DIFFERENTIAL STAINING OF CARTILAGE AND BONE IN WHOLE MOUSE FETUSES BY ALCIAN-BLUE AND ALIZARIN-RED-S
DIFFERENTIAL STAINING OF CARTILAGE AND BONE IN WHOLE MOUSE FETUSES BY ALCIAN-BLUE AND ALIZARIN-RED-S
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DOI：
10.1002/tera.1420220306
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
MCLEOD, MJ
中科院分区：
文献类型：
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作者：
MCLEOD, MJ
Inouye描述的用于对足月小鼠胎儿骨骼进行染色的程序适用于妊娠14-18天的小鼠胚胎和胎儿。对于较年轻的样品的主要适应包括在丙酮中的较长时间，代替结皮，以及省略KOH水溶液步骤。这些调整需要更多的时间，但结果是完整标本的染色始终良好。
The procedure described by Inouye for the staining of full term mouse fetal skeletons was adapted for use with mouse embryos and fetuses of 14-18 days of gestation. The main adaptations for younger specimens involve a longer time in acetone, in lieu of skinning, and omission of the aqueous KOH step. These adaptations require more time but result in consistently good staining of intact specimens.