Fluorescence spectroscopy and microscopy as tools for monitoring redox transformations of uranium in biological systems.
Fluorescence spectroscopy and microscopy as tools for monitoring redox transformations of uranium in biological systems.
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DOI：
10.1039/c5sc00661a
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发表时间：
2015-09-01
期刊：
影响因子：
8.4
通讯作者：
Natrajan LS
中科院分区：
文献类型：
--
作者：
Jones DL;Andrews MB;Swinburne AN;Botchway SW;Ward AD;Lloyd JR;Natrajan LS
发光光谱，显微镜和寿命图像映射提供了新的见解Geetriumsulfreducens与铀酰的生物还原。我们报告的氧化还原反应的铀在模型条件下，使用发光光谱学的研究，其方便性和广泛的可用性有可能提供新的见解，特别感兴趣的生物修复策略-酶还原UVIO 2 2+的细菌，如Geophylsulfurreducens。UVIO 2 2+的固有发光特性已与共聚焦荧光显微镜技术和寿命图像映射相结合，以直接报告铀生物还原过程中细胞系统中的铀浓度、定位和氧化状态，表明铀酰物质在细胞膜表面的定位起着重要的作用，并且在早期阶段，细胞外生物源特征与铀酰吸附的细胞物质一起形成的生物还原。使用共聚焦显微镜串联寿命图像映射提供了改进的时间和空间分辨率（纳秒至微秒和亚微米分别）比更传统的X射线为基础的技术，并提供了潜在的图像氧化还原反应发生在原位。总之，这些技术提供了一个很好的和敏感的探针，以评估在生物还原过程中，目前正在考虑的氧化还原活性放射性核素的污染土地的修复策略的铀的协调环境。
Luminescence spectroscopy, microscopy and lifetime image mapping offers new insights into the bioreduction of Geobacter sulfurreducens with uranyl. We report a study of redox reactions of uranium in model conditions using luminescence spectroscopy, which with its ease and wide availability has the potential to offer new insights into a bioremediation strategy of particular interest – the enzymatic reduction of UVIO2 2+ by bacteria such as Geobacter sulfurreducens. The inherent luminescent properties of UVIO2 2+ have been combined with confocal fluorescence microscopy techniques and lifetime image mapping to report directly on uranium concentration, localisation and oxidation state in cellular systems during uranium bioreduction, suggesting that localisation of uranyl species on the cell membrane surface plays an important role and that extracellular biogenic features form alongside uranyl sorbed cellular species during early stages of the bioreduction. The use of confocal microscopy in tandem with lifetime image mapping offers both improved temporal and spatial resolution (nanoseconds to microseconds and sub-micron respectively) than more conventional X-ray based techniques and offers the potential to image redox reactions occurring in situ. Together, these techniques provide an excellent and sensitive probe to assess the coordination environment of uranium during bioreduction processes that are currently being considered for remediation strategies of redox active radionuclides present in contaminated land.
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