Reactivity of 2'-deoxyoxanosine, a novel DNA lesion.
Reactivity of 2'-deoxyoxanosine, a novel DNA lesion.
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2-脱氧氧核苷（一种新型 DNA 损伤）的反应性。
DOI：
10.1093/nass/42.1.7
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
K. Makino
中科院分区：
文献类型：
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作者：
T. Suzuki;M. Yamada;T. Ishida;T. Morii;K. Makino
2'-脱氧氧鸟苷 (dOxo) 是一种新型 DNA 损伤，由 2'-脱氧鸟苷与亚硝酸或一氧化氮反应产生。我们发现 dOxo 在生理条件下与甘氨酸发生反应。通过光谱数据鉴定该产物为 dOxo 的六元环与甘氨酸的氨基之间的加合物。该加合物在生理条件下比 dOxo 更稳定。含有 dOxo 的寡脱氧核苷酸与甘氨酸的温育也产生加合物。这些结果表明，DNA 中形成的 dOxo 在体内与 DNA 周围各种化合物的氨基反应，形成加合物。
2'-Deoxyoxanosine (dOxo) is a novel DNA lesion produced from 2'-deoxyguanosine by the reaction with nitrous acid or nitric oxide. We found that dOxo reacted with glycine under physiological conditions. The product was identified by spectrometric data as an adduct between the six membered ring of dOxo and an amino group of glycine. The adduct was more stable than dOxo under physiological conditions. The incubation of an oligodeoxynucleotide containing dOxo with glycine gave also rise to the adduct. These results suggest that dOxo formed in DNA reacts with amino groups of various compounds around DNA in vivo resulting in the adduct.