Simultaneous visualization of tumour oxygenation, neovascularization and contrast agent perfusion by real-time three-dimensional optoacoustic tomography
Simultaneous visualization of tumour oxygenation, neovascularization and contrast agent perfusion by real-time three-dimensional optoacoustic tomography
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DOI：
10.1007/s00330-015-3980-0
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发表时间：
2016-06-01
影响因子：
5.9
通讯作者：
Razansky, Daniel
中科院分区：
文献类型：
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作者：
Ermolayev, Vladimir;Dean-Ben, Xose Luis;Razansky, Daniel
异质实体肿瘤的活体成像对于了解血液灌注谱非常重要，这些血流灌注谱负责建立肿瘤肿块内的多个参数，如缺氧和营养梯度、细胞活力、增殖和药物反应电位。在此，我们开发了一种基于体积多光谱光声断层成像（vMSOT）的方法，用于临床前模型的癌症成像，并探索其实时三维成像解剖、血管和功能肿瘤的能力。与基于横断面（2D）图像采集作为3D渲染基础的方法相比，vMSOT以每秒10个体积帧的速度获得了整个肿瘤体积的并发观察。这种真正的四维成像性能使我们能够同时评估实性乳腺肿瘤中的血氧梯度和血管化，并揭示体内不同类型的血液灌注谱。新引入的高分辨率三维快速肿瘤灌注跟踪能力表明vMSOT在临床前癌症研究和治疗中是一种强有力的方法。由于成像装置可以在固定和手持模式下同样操作，因此该解决方案很容易用于临床环境中的灌注监测。vMSOT实时可视化三维解剖、血管和功能肿瘤。乳腺肿瘤模型显示三种类型的血流灌注谱。该方法易于适应在手持临床模式下操作。
Intravital imaging within heterogenic solid tumours is important for understanding blood perfusion profiles responsible for establishment of multiple parameters within the tumour mass, such as hypoxic and nutrition gradients, cell viability, proliferation and drug response potentials.Herein, we developed a method based on a volumetric multispectral optoacoustic tomography (vMSOT) for cancer imaging in preclinical models and explored its capacity for three-dimensional imaging of anatomic, vascular and functional tumour profiles in real time.In contrast to methods based on cross-sectional (2D) image acquisition as a basis for 3D rendering, vMSOT has attained concurrent observations from the entire tumour volume at 10 volumetric frames per second. This truly four dimensional imaging performance has enabled here the simultaneous assessment of blood oxygenation gradients and vascularization in solid breast tumours and revealed different types of blood perfusion profiles in-vivo.The newly introduced capacity for high-resolution three-dimensional tracking of fast tumour perfusion suggests vMSOT as a powerful method in preclinical cancer research and theranostics. As the imaging setup can be equally operated in both stationary and handheld mode, the solution is readily translatable for perfusion monitoring in a clinical setting.aEuro cent vMSOT visualizes 3D anatomic, vascular and functional tumour profiles in real time.aEuro cent Three types of blood perfusion profiles are revealed in breast tumour model.aEuro cent The method is readily adaptable to operate in a handheld clinical mode.