Culture-independent analysis of the gut microbiota in colorectal cancer and polyposis
Culture-independent analysis of the gut microbiota in colorectal cancer and polyposis
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DOI：
10.1111/j.1462-2920.2007.01503.x
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发表时间：
2008-03-01
影响因子：
5.1
通讯作者：
Marchesi, Julian R.
中科院分区：
文献类型：
--
作者：
Scanlan, Pauline D.;Shanahan, Fergus;Marchesi, Julian R.
肠道微生物群的作用通常被认为是结直肠癌发生和发展的潜在病因因素。由于肠道中的大多数细菌难以培养，我们使用非培养方法在患有结直肠癌和腺瘤性息肉病的受试者中研究了这种生态系统，这些受试者具有患结直肠癌的高风险。选择20例结直肠癌和20例息肉切除志愿者进行分析。利用16S rRNA基因变性梯度凝胶电泳和核糖体基因间间隔区分析（RISA）对优势菌和几个细菌亚群的多样性和时间稳定性进行了探索。还进行了远端肠道微生物群环境的代谢组学分析。明显的是，患有结直肠癌和息肉病的受试者的微生物群的时间稳定性显著降低，多样性增加。结果表明，在该地区，纤细梭菌和梭菌的多样性显著增加.在两个疾病组中也观察到球孢子虫亚组。与对照志愿者相比，在结直肠癌和息肉切除受试者中观察到代谢组的明显划分。与对照组相比，结肠直肠癌和息肉切除受试者的肠道微生物群及其代谢产物均发生显著变化。
A role for the intestinal microbiota is routinely cited as a potential aetiological factor in colorectal cancer initiation and progression. As the majority of bacteria in the gut are refractory to culture we investigated this ecosystem in subjects with colorectal cancer and with adenomatous polyposis who are at high risk of developing colorectal cancer, using culture-independent methods. Twenty colorectal cancer and 20 polypectomized volunteers were chosen for this analysis. An exploration of the diversity and temporal stability of the dominant bacteria and several bacterial subgroups was undertaken using 16S rRNA gene denaturing gradient gel electrophoresis and ribosomal intergenic spacer analysis (RISA). Metabonomic analysis of the distal gut microbiota's environment was also undertaken. A significantly reduced temporal stability and increased diversity for the microbiota of subjects with colorectal cancer and polyposis was evident. A significantly increased diversity of the Clostridium leptum and C. coccoides subgroups was also noted for both disease groups. A clear division in the metabonome was observed for the colorectal cancer and polypectomized subjects compared with control volunteers. The intestinal microbiota and their metabolites are significantly altered in both colorectal cancer and polypectomized subjects compared with controls.