Site-specific protein modification by genetic encoded disulfide compatible thiols
Site-specific protein modification by genetic encoded disulfide compatible thiols
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通过基因编码的二硫键相容性硫醇进行位点特异性蛋白质修饰
DOI：
10.1016/j.cclet.2019.04.075
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发表时间：
2020
影响因子：
9.1
通讯作者：
Tao Liu
中科院分区：
文献类型：
--
作者：
Xinyu Ling;Heqi Chen;Wei Zheng;Liying Chang;Yong Wang;Tao Liu
Cysteine chemistry provides a low cost and convenient way for site-specific protein modification. However, recombinant expression of disulfide bonding containing protein with unpaired cysteine is technically challenging and the resulting protein often suffers from significantly reduced yield and activity. Here we used genetic code expansion technique to introduce a surface exposed self-paired di-thiol functional group into proteins, which can be selectively reduced to afford active thiols. Two compounds containing self-paired disulfides were synthesized, and their genetic incorporations were validated using green fluorescent proteins (GFP). The compatibility of these self-paired di-thiols with natural disulfide bond was demonstrated using antibody fragment to afford site-specifically labeled antibody. This work provides another valuable building block into the chemical tool-box for site-specific labeling of proteins containing internal disulfides.
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影响因子：
8.4
作者：
Akkapeddi P;Azizi SA;Freedy AM;Cal PMSD;Gois PMP;Bernardes GJL
通讯作者：
Bernardes GJL
影响因子：
1.6
作者：
Ishita Sengupta;J. Udgaonkar
通讯作者：
Ishita Sengupta;J. Udgaonkar
DOI：
10.1073/pnas.1605363113
发表时间：
2016-05-24
影响因子：
11.1
作者：
Liu, Tao;Wang, Yan;Schultz, Peter G.
通讯作者：
Schultz, Peter G.
影响因子：
9.1
作者：
Y. Pu;Hui Yuan;Ming-Hsiu Yang;B. He;Z. Gu
通讯作者：
Y. Pu;Hui Yuan;Ming-Hsiu Yang;B. He;Z. Gu
影响因子：
--
作者：
Yanagisawa, Tatsuo;Ishii, Ryohei;Yokoyama, Shigeyuki
通讯作者：
Yokoyama, Shigeyuki