Genome sequencing reveals agronomically important loci in rice using MutMap
Genome sequencing reveals agronomically important loci in rice using MutMap
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DOI：
10.1038/nbt.2095
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发表时间：
2012-02-01
影响因子：
46.9
通讯作者：
Terauchi, Ryohei
中科院分区：
文献类型：
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作者：
Abe, Akira;Kosugi, Shunichi;Terauchi, Ryohei
大多数农艺性状受多个基因控制，这些基因引起较小的表型效应，使得这些基因的鉴定变得困难。在这里，我们介绍MutMap，一种基于全基因组重测序的方法，从一个分离的植物群体，显示一个有用的表型的汇集DNA。在MutMap中，突变体直接与原始野生型品系杂交，然后自交，允许在第二代子代（F-2）后代中明确分离细微的表型差异。这种方法特别适用于作物物种，因为它使所需的遗传杂交（n = 1或0）和突变体F-2后代的数量最小化。我们将MutMap应用于日本优良水稻品种的7个突变体，并确定了最有可能携带突变的独特基因组位置，这些突变导致淡绿色叶和半矮生，这是一种农艺学相关性状。这些结果表明MutMap可以加速水稻和其他作物的遗传改良。
The majority of agronomic traits are controlled by multiple genes that cause minor phenotypic effects, making the identification of these genes difficult. Here we introduce MutMap, a method based on whole-genome resequencing of pooled DNA from a segregating population of plants that show a useful phenotype. In MutMap, a mutant is crossed directly to the original wild-type line and then selfed, allowing unequivocal segregation in second filial generation (F-2) progeny of subtle phenotypic differences. This approach is particularly amenable to crop species because it minimizes the number of genetic crosses (n = 1 or 0) and mutant F-2 progeny that are required. We applied MutMap to seven mutants of a Japanese elite rice cultivar and identified the unique genomic positions most probable to harbor mutations causing pale green leaves and semidwarfism, an agronomically relevant trait. These results show that MutMap can accelerate the genetic improvement of rice and other crop plants.