Poxviruses Encode a Reticulon-Like Protein that Promotes Membrane Curvature.
Poxviruses Encode a Reticulon-Like Protein that Promotes Membrane Curvature.
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DOI：
10.1016/j.celrep.2016.01.075
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发表时间：
2016-03-08
期刊：
影响因子：
8.8
通讯作者：
Moss B
中科院分区：
文献类型：
--
作者：
Erlandson KJ;Bisht H;Weisberg AS;Hyun SI;Hansen BT;Fischer ER;Hinshaw JE;Moss B
痘病毒是被包膜的DNA病毒，在细胞质内复制。第一个病毒结构是新月形和球形颗粒，具有被认为来自内质网（ER）的脂蛋白膜双层。我们发现，A17是一种保守的病毒跨膜蛋白，对新月形成至关重要，它与细胞网状样蛋白形成同源寡聚物，并具有相同的拓扑特征，从而促进膜曲率并有助于内质网的管状结构。将纯化的A17蛋白加入脂质体中，在A17浓度低和浓度高时分别形成直径为25 nm的囊泡和小管。此外，在缺乏其他病毒结构蛋白的情况下，A17的细胞内表达将内质网转化为聚集的三维管状网络。我们认为A17是一种病毒网状样蛋白，在痘病毒膜的生物发生过程中有助于弯曲。
Poxviruses are enveloped DNA viruses that replicate within the cytoplasm. The first viral structures are crescents and spherical particles with a lipoprotein membrane bilayer thought to be derived from the endoplasmic reticulum (ER). We determined that A17, a conserved viral transmembrane protein essential for crescent formation, forms homo-oligomers and shares topological features with cellular reticulon-like proteins, which promote membrane curvature and contribute to the tubular structure of the ER. When the purified A17 protein was incorporated into liposomes, 25 nm diameter vesicles and tubules formed at low and high A17 concentrations, respectively. In addition, intracellular expression of A17, in the absence of other viral structural proteins, transformed the ER into aggregated 3-dimensional tubular networks. We suggest that A17 is a viral reticulon-like protein that contributes to curvature during biogenesis of the poxvirus membrane.