Molecular based evidence for a lack of gene-flow between Rosaxhybrida and wild Rosa species in Japan
Molecular based evidence for a lack of gene-flow between Rosaxhybrida and wild Rosa species in Japan
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DOI：
10.5511/plantbiotechnology.10.1217a
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发表时间：
2011-01-01
影响因子：
1.6
通讯作者：
Tanaka, Yoshikazu
中科院分区：
文献类型：
--
作者：
Nakamura, Noriko;Tems, Ursula;Tanaka, Yoshikazu
评估引进转基因植物对环境的潜在影响的一个重要部分是评估转基因植物向相关野生物种的基因流动潜力。在评估基因是否可能流向野生物种的风险过程中，需要这一信息。评估基因流动的一种方法是使用分子技术来鉴定野生物种种群中可能起源于栽培物种的基因。该方法的优点是不需要表型标记或性状来测量基因流。本研究对三种野生蔷薇属植物（R.何首乌，R.光叶藓还有法国来自Crep。和R. rugosa Thunb.）从日本各地的几个地方选择，其中野生玫瑰生长在接近栽培玫瑰植物（Rosaxhybrida）的地方。为了确定是否发生了来自栽培玫瑰的基因流，通过PCR分析了来自野生玫瑰植物的1，296个幼苗的幼叶中KSN基因座的存在。该基因座起源于一个运动的R。中国花变种spontanea（spontanea）et Wils.）Yu et Ku和参与观察到的栽培玫瑰杂交种的经常性开花表型，但在日本种玫瑰中不存在。KSN基因座是不存在的，在所有的幼苗采样，表明没有基因流到野生蔷薇品种的栽培玫瑰已经发生，并提供证据表明，在日本栽培野生蔷薇品种的基因流的概率很低或不存在。
An important part of the assessment of the potential environmental impact from the introduction of a genetically modified (GM) plant is an evaluation of the potential for gene flow from the GM plant to related wild species. This information is needed as part of the risk-assessment process, in the context of whether gene flow to wild species is possible. One method for evaluating gene flow is to use molecular techniques to identify genes in wild species populations that may have originated from a cultivated species. An advantage of this method is that a phenotypic marker or trait is not required to measure gene flow. In the present study we analyzed the seedlings of seeds from three wild native Rosa species (R. multiflora Thunb., R. luciae Rochebr. et Franch. ex Crep. and R. rugosa Thunb.) selected from several locations across Japan where the wild rose was growing in close proximity to cultivated rose plants (Rosaxhybrida). To determine whether gene flow from cultivated rose had occurred, young leaves of 1,296 seedlings from the wild Rosa plants were analyzed by PCR for the presence of the KSN locus. This locus originated from a sport of R. chinensis Jacq. var. spontanea (Rehd. et Wils.) Yu et Ku and is involved in the recurrent flowering phenotype observed for cultivated rose hybrids, but is absent in Japanese species roses. The KSN locus was absent in all seedlings sampled, indicating no gene flow to wild Rosa species from the cultivated rose had occurred, and providing evidence that the probability of gene flow from cultivated to wild Rosa species in Japan is low or non-existent.