Nutrient Assimilation by First‐Feeding African Catfish, Clarias gariepinus, Assessed Using Stable Isotope Analysis
Nutrient Assimilation by First‐Feeding African Catfish, Clarias gariepinus, Assessed Using Stable Isotope Analysis
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使用稳定同位素分析评估首次喂养的非洲鲶鱼 Clarias gariepinus 的营养同化
DOI：
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发表时间：
2013
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影响因子：
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通讯作者：
J. Vielma
中科院分区：
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作者：
U. Enyidi;M. Kiljunen;Roger I. Jones;J. Pirhonen;J. Vielma
对受试鱼类的成分同化和生物量贡献的知识在饲料配方中很重要。用~(13)C和~(15)N的稳定同位素研究了在30d的试验中，在首次投喂的非洲胡子叉尾鱼(Clarias Gariepinus)的FM替代饲料中，Bambaranut、Voandzeia subterranea、Meal(BNM)、玉米、Zea Mays、Meal(CM)和Fm(Fm)的同化和生物量贡献。在L三次重复投放的玻璃水族箱中投放40尾/15尾。幼虫饲喂不同的饲料：FM、BNM和CM。试验日粮中FM ： BNM ： CM的比例为：饲料1(F1)60：20：20，饲料2(F2)40：40：20，饲料3(F3)20：60：20，饲料4(F4)20：20：60。对δ13C和δ15N的饲料和幼虫稳定同位素进行了分析。仔鱼的特定生长率(SGR)高，促进了饲料中δ13C和δ15N的掺入。投喂F1和F2组的SGR最高，投喂F3和F4组的SGR显著低于投喂F3和F4组。δ13C和δ15N分析结果表明，在相同包裹体水平下，FM对生物量的贡献与BNM相似，均好于CM。每种成分对幼虫体细胞粗蛋白的氮贡献随着成分包裹量和同化量的增加而增加。稳定同位素分析有助于分析养分的同化和利用。
Knowledge of ingredients assimilation and biomass contribution to recipient fish is important in feed formulation. The stable isotopes of 13C and 15N were used to investigate the assimilation and biomass contribution of bambaranut, Voandzeia subterranea, meal (BNM), corn, Zea mays, meal (CM) and fish meal (FM), in FM substituted diets of first feeding African catfish, Clarias gariepinus, during a 30-d experiment. The catfish larvae were stocked at 40 fish/15 L three replicate glass aquaria. Larvae were fed with experimental diets varying FM, BNM, and CM. Proportions of FM : BNM : CM in the experimental diets were: feed 1 (F1) 60:20:20; feed 2 (F2) 40:40:20; feed 3 (F3) 20:60:20; and feed 4 (F4) 20:20:60. Feeds and larval stable isotopes of δ13C and δ15N were analyzed. Larval specific growth rates (SGRs) were high, enhancing incorporation of dietary δ13C and δ15N. The SGR was highest for catfish fed F1 and F2 but significantly lower for those on F3 and F4. Results of δ13C and δ15N analyses showed that the biomass contribution from FM was similar to BNM, which were better than CM at equal inclusion levels. The nitrogen contribution per ingredient to larval somatic crude protein was increasing with increases in ingredient inclusion and assimilation. Stable isotope analysis is useful for analyzing assimilation and utilization of nutrients.