Rosmarinic acid is a homoserine lactone mimic produced by plants that activates a bacterial quorum-sensing regulator
Rosmarinic acid is a homoserine lactone mimic produced by plants that activates a bacterial quorum-sensing regulator
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DOI：
10.1126/scisignal.aaa8271
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发表时间：
2016-01-05
影响因子：
7.3
通讯作者：
Krell, Tino
中科院分区：
文献类型：
--
作者：
Corral-Lugo, Andres;Daddaoua, Abdelali;Krell, Tino
群体感应是一种细菌通信机制，它控制基因，使细菌能够作为群落生活，例如生物膜。高丝氨酸内酯(HSL)分子是革兰氏阴性菌群体感应信号。植物也会产生以前未知的化合物，影响群体感应。我们确定迷迭香酸是一种植物衍生的化合物，其功能类似于HSL。体外实验表明，迷迭香酸与铜绿假单胞菌PAO1的群体敏感调节因子RhlR结合，并与细菌配体N-丁酰-高丝氨酸内酯(C4-HSL)竞争。此外，迷迭香酸在体外对RhlR介导的转录的刺激作用强于C4-HSL。在铜绿假单胞菌中，迷迭香酸诱导了群体感应相关基因的表达，增加了生物膜的形成和毒力因子绿青素和弹力酶的产生。由于铜绿假单胞菌PAO1侵染诱导植物根分泌迷迭香酸，我们的结果表明迷迭香酸的分泌是植物刺激过早的量子感应反应的一种防御机制。铜绿假单胞菌是一种普遍存在的感染动植物的病原体；因此，确定迷迭香酸是早熟群体感应反应的诱导剂可能在农业中有用，并为人类的治疗策略提供参考。
Quorum sensing is a bacterial communication mechanism that controls genes, enabling bacteria to live as communities, such as biofilms. Homoserine lactone (HSL) molecules function as quorum-sensing signals for Gram-negative bacteria. Plants also produce previously unidentified compounds that affect quorum sensing. We identified rosmarinic acid as a plant-derived compound that functioned as an HSL mimic. In vitro assays showed that rosmarinic acid bound to the quorum-sensing regulator RhlR of Pseudomonas aeruginosa PAO1 and competed with the bacterial ligand N-butanoyl-homoserine lactone (C4-HSL). Furthermore, rosmarinic acid stimulated a greater increase in RhlR-mediated transcription in vitro than that of C4-HSL. In P. aeruginosa, rosmarinic acid induced quorum sensing-dependent gene expression and increased biofilm formation and the production of the virulence factors pyocyanin and elastase. Because P. aeruginosa PAO1 infection induces rosmarinic acid secretion from plant roots, our results indicate that rosmarinic acid secretion is a plant defense mechanism to stimulate a premature quorumsensing response. P. aeruginosa is a ubiquitous pathogen that infects plants and animals; therefore, identification of rosmarinic acid as an inducer of premature quorum-sensing responses may be useful in agriculture and inform human therapeutic strategies.