An endogenous bornavirus‐like nucleoprotein in miniopterid bats retains the RNA‐binding properties of the original viral protein
An endogenous bornavirus‐like nucleoprotein in miniopterid bats retains the RNA‐binding properties of the original viral protein
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DOI：
10.1002/1873-3468.14290
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发表时间：
2022-01
期刊：
影响因子：
3.5
通讯作者：
Yahiro Mukai;Masayuki Horie;Shohei Kojima;Junna Kawasaki;Ken Maeda;K. Tomonaga
中科院分区：
文献类型：
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作者：
Yahiro Mukai;Masayuki Horie;Shohei Kojima;Junna Kawasaki;Ken Maeda;K. Tomonaga
内源性博尔纳病毒样核蛋白元件（EBLN）是源自脊椎动物基因组中博尔纳病毒N基因的序列。一些EBLN已被建议在宿主细胞中编码功能蛋白，然而，很少有人知道它们的进化和功能的关系，从EBLN起源的病毒基因。在这里，我们基于N作为RNA结合蛋白的性质预测了EBLN的功能。我们发现，在miniopterid蝙蝠中的EBLN（miEBLN-1）已经在纯化选择下进化，并编码一种RNA结合蛋白（miEBLN-1 p），其生化特性与博纳病毒N相似。此外，我们发现miEBLN-1 p与宿主RNA结合蛋白（例如MOV 10）相互作用。这些数据表明，miEBLN-1 p已被认为是一种RNA结合蛋白，具有与miniopterid蝙蝠中的外源博纳病毒N相似的性质。
Endogenous bornavirus‐like nucleoprotein elements (EBLNs) are sequences derived from bornaviral N genes in vertebrate genomes. Some EBLNs have been suggested to encode functional proteins in host cells; however, little is known about their evolution and functional relationship to the viral genes from which EBLNs originate. Here, we predicted functionality of EBLNs based on the properties of N as an RNA‐binding protein. We showed an EBLN in miniopterid bats (miEBLN‐1) has evolved under purifying selection and encodes an RNA‐binding protein (miEBLN‐1p) with biochemical properties similar to bornaviral N. Furthermore, we revealed miEBLN‐1p interacts with host RNA‐binding proteins, such as MOV10. These data suggest that miEBLN‐1p has been exapted as an RNA‐binding protein with similar properties to exogenous bornaviral N in miniopterid bats.