Genotoxicity and Estrogenic Activity of 3,3′-Dinitrobisphenol A in Goldfish
Genotoxicity and Estrogenic Activity of 3,3′-Dinitrobisphenol A in Goldfish
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DOI：
10.1271/bbb.80193
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发表时间：
2008-08
期刊：
影响因子：
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通讯作者：
Tomoyasu Toyoizumi;Y. Deguchi;S. Masuda;N. Kinae
中科院分区：
文献类型：
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作者：
Tomoyasu Toyoizumi;Y. Deguchi;S. Masuda;N. Kinae
3，3‘-二硝基双酚A(Ditro-BPA)是双酚A(BPA)和亚硝酸盐在酸性条件下生成的产物。它对雄性ICR小鼠的微核试验显示出遗传毒性，但其雌激素活性尚未在体内检测。以金鱼(Carassius Auratus)为实验动物，采用酶联免疫吸附试验(EL ISA)测定其血浆卵黄蛋白原(VTG)水平，以检测其雌激素样活性。给金鱼腹腔注射10 mg/kg体重的二硝基双酚A后，血浆中VTG的表达没有增加。我们还用单细胞凝胶电泳法(彗星试验)和金鱼微核试验检测了二硝基BPA的遗传毒性。腹腔注射二硝基双酚A后，小鼠血细胞DNA尾矩增加。相同剂量的二硝基双酚A可显著提高金鱼鳃微核发生率。另一方面，双酚A并未显著增加微核率。综上所述，我们发现二硝基BPA没有雌激素样活性，但具有比BPA更强的遗传毒性。
3,3′-Dinitrobisphenol A (dinitro-BPA) is formed in a mixture of bisphenol A (BPA) and nitrite under acidic conditions. It shows genotoxicity in male ICR mice on a micronucleus test, but its estrogenic activity has not been examined in vivo. We examined its estrogenic activity using goldfish (Carassius auratus) by measuring plasma levels of vitellogenin (VTG) by the ELISA method. Expression of VTG didn’t increase in the plasma of goldfish intraperitoneal injected with dinitro-BPA at a dose of 10 mg/kg of body weight. We also examined the genotoxicity of dinitro-BPA by single-cell gel electrophoresis (comet assay) and a micronucleus test using goldfish. The DNA tail moment of blood cells increased after intraperitoneal injection of dinitro-BPA. Dinitro-BPA at the same dose significantly increased micronucleus frequency in gills of goldfish. On the other hand, BPA did not significantly increase the frequency of micronucleated cells. In conclusion, we found that dinitro-BPA did not show estrogenic activity, but had genotoxic potency stronger than that of BPA.