Further development of a recombinant feline herpesvirus type 1 vector expressing feline calicivirus immunogenic antigen.
Further development of a recombinant feline herpesvirus type 1 vector expressing feline calicivirus immunogenic antigen.
复制标题
进一步开发表达猫杯状病毒免疫原性抗原的重组猫疱疹病毒 1 型载体。
DOI：
10.1292/jvms.60.717
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
T. Mikami
中科院分区：
文献类型：
--
作者：
N. Yokoyama;K. Fujita;A. Damiani;E. Sato;K. Kurosawa;T. Miyazawa;S. Ishiguro;M. Mochizuki;K. Maeda;T. Mikami
本研究以猫疱疹病毒1型（FHV-1）胸苷激酶（TK）缺失突变株（C7301 dlTK）为研究对象，在其TK缺失位点插入猫钙化病毒（FCV）前体衣壳蛋白基因，构建了重组FHV-1（C7301 dlTK-Cap）。在本研究中，我们构建了进一步改进的重组FHV-1（dlTK（gCp）-Cap），其在C7301 dlTK-Cap的FCV前体衣壳基因上游携带推定的FHV-1 gC启动子序列。细胞培养物中dlTK（gCp）-Cap的生长动力学与C7301 dlTK和C7301 dlTK-Cap相似。通过间接免疫荧光和酶联免疫吸附测定证实了dlTK（gCp）-Cap对FCV免疫原性抗原的强表达。此外，用dlTK（gCp）-Cap的一次疫苗接种保护猫比用C7301 dlTK-Cap更有效地抵抗随后的强毒FCV攻击。
We previously reported the attenuation of thymidine kinase (TK) deficient mutant (C7301dlTK) of feline herpesvirus type 1 (FHV-1) in cats and the construction of a recombinant FHV-1 (C7301dlTK-Cap) inserted a precursor capsid gene of feline calcivirus (FCV) into the TK deletion locus of the C7301dlTK. In this study, we constructed a further improved recombinant FHV-1 (dlTK(gCp)-Cap) carrying a putative FHV-1 gC promoter sequence upstream of the FCV precursor capsid gene of the C7301dlTK-Cap. Growth kinetics of the dlTK(gCp)-Cap in cell cultures was similar to those of C7301dlTK and C7301dlTK-Cap. A strong expression of FCV immunogenic antigen by dlTK(gCp)-Cap was confirmed by indirect immunofluorescence and enzyme-linked immunosorbent assays. In addition, one vaccination with dlTK(gCp)-Cap protected cats more effective against subsequent virulent FCV challenge than that with C7301dlTK-Cap.