Photosynthetic Growth and Energy Conversion in an Engineered Phototroph Containing Thermochromatium tepidum Light-Harvesting Complex 1 and the Rhodobacter sphaeroides Reaction Center Complex
Photosynthetic Growth and Energy Conversion in an Engineered Phototroph Containing Thermochromatium tepidum Light-Harvesting Complex 1 and the Rhodobacter sphaeroides Reaction Center Complex
复制标题
含有 Thermochromatium tepidum 光捕获复合物 1 和球形红杆菌反应中心复合物的工程光养生物的光合生长和能量转换
DOI：
10.1021/acs.biochem.1c00462
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发表时间：
2021
期刊：
影响因子：
2.9
通讯作者：
Wang-Otomo Zheng-Yu
中科院分区：
文献类型：
--
作者：
Nagashima Kenji V. P.;Nagashima Sakiko;Kitashima Masaharu;Inoue Kazuhito;Madigan Michael T.;Kimura Yukihiro;Wang-Otomo Zheng-Yu
嗜热紫色硫细菌（thermochromatium tepidumum）的光收集复合物1 （LH1）可以在紫色非硫细菌（球形rhodobacter sphereroides）中表达，并与原生verba形成功能性RC-LH1复合物。球体反应中心（Nagashima, k.v.p，等）。国家的。学会科学。u.s. .2017, 114, 10906−10911)。尽管两者之间有很大的上坡能量差距。tepidumLH1和theRba。在这个嵌合物中，已经证明光能可以转移，与在原生体中看到的一致。sphaeroidesRC-LH1复杂。在本研究中，该嵌合复合体对杂交生物生长和光合能量转换的贡献被量化。合成该嵌合物的突变体在被tch优先吸收的940 nm发光二极管（LED）光下进行光养生长。tepidumLH1和rba的突变株在该波长的低强度下表现出更快的生长。球状体缺乏LH2，突变体缺乏所有的光收集复合物。当在850 nm的LED光下生长时，含有原生藻的菌株。sphaeroidesLH1-RC生长速度快于嵌合菌株。从RC到膜整合的细胞色素bc1复合体的电子转移也通过闪诱导血红素的吸收变化来估计。在嵌合菌株中，泛素通过LH1环结构的转运速率与本地菌株几乎相同。sphaeroides。我们的结论是。tepidumLH1可以在rba中发挥天然LH1的生理功能。sphaeroides。
Light-harvesting complex 1 (LH1) of the thermophilic purple sulfur bacteriumThermochromatium tepidumcan be expressed in the purple non-sulfur bacteriumRhodobacter sphaeroidesand forms a functional RC-LH1 complex with the nativeRba. sphaeroidesreaction center (Nagashima, K. V. P., et al.Proc. Natl. Acad. Sci. U. S. A.2017, 114, 10906−10911). Although there is a large uphill energy gap betweenTch. tepidumLH1 and theRba. sphaeroidesRC in this chimeric complex, it has been shown that light energy can be transferred, consistent with that seen in the nativeRba. sphaeroidesRC-LH1 complex. In this study, the contribution of this chimeric complex to growth and photosynthetic energy conversion in the hybrid organism was quantified. The mutant synthesizing this chimeric complex was grown phototrophically under 940 nm light-emitting diode (LED) light preferentially absorbed byTch. tepidumLH1 and showed faster growth at low intensities of this wavelength than both a mutant strain ofRba. sphaeroideslacking LH2 and a mutant lacking all light-harvesting complexes. When grown with 850 nm LED light, the strain containing the nativeRba. sphaeroidesLH1-RC grew faster than the chimeric strain. Electron transfer from the RC to the membrane-integrated cytochromebc1complex was also estimated by flash-induced absorption changes in hemeb. The rate of ubiquinone transport through the LH1 ring structure in the chimeric strain was virtually the same as that in nativeRba. sphaeroides. We conclude thatTch. tepidumLH1 can perform the physiological functions of native LH1 inRba. sphaeroides.