DEVELOPMENT OF BACULOVIRUS TRIPLE AND QUADRUPLE EXPRESSION VECTORS - COEXPRESSION OF 3 OR 4 BLUETONGUE VIRUS PROTEINS AND THE SYNTHESIS OF BLUETONGUE VIRUS-LIKE PARTICLES IN INSECT CELLS
DEVELOPMENT OF BACULOVIRUS TRIPLE AND QUADRUPLE EXPRESSION VECTORS - COEXPRESSION OF 3 OR 4 BLUETONGUE VIRUS PROTEINS AND THE SYNTHESIS OF BLUETONGUE VIRUS-LIKE PARTICLES IN INSECT CELLS
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DOI：
10.1093/nar/21.5.1219
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发表时间：
1993-03-11
影响因子：
14.9
通讯作者：
ROY, P
中科院分区：
文献类型：
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作者：
BELYAEV, AS;ROY, P
杆状病毒多基因转移载体 pAcAB3 和 pAcAB4 已被开发出来，可通过单次共转染实验将三个或四个外源基因分别插入苜蓿银纹夜蛾核型多角体病毒 (AcNPV) 基因组中。 pAcAB3载体包含一个多角体蛋白启动子和两个位于多角体蛋白启动子两侧但方向相反的p10启动子。 pAcAB4 载体具有与第一个拷贝方向相反的额外多角体启动子，该启动子与第一个 p10 启动子并置。这些衍生载体（pAcAB3、pAcAB4）均已分别用于同时表达三个或四个蓝舌病毒（BTV）基因。当表达BTV 4个主要结构基因的重组病毒(AcBT-3/2/7/5)感染草地贪夜蛾细胞时，组装了由BTV的VP2、VP3、VP5和VP7组成的双衣壳病毒样颗粒。
Baculovirus multiple gene transfer vectors pAcAB3 and pAcAB4 have been developed to facilitate the insertion of three or four foreign genes respectively into the Autographa californica nuclear polyhedrosis virus (AcNPV) genome by a single co-transfection experiment. The pAcAB3 vector contains a polyhedrin promoter and two p10 promoters on either side of the polyhedrin promoter but in opposite orientations. The pAcAB4 vector has an additional polyhedrin promoter in opposite orientation to the first copy that is in juxtaposition to the first p10 promoter. Each of these derived vectors (pAcAB3, pAcAB4) have been used for the simultaneous expression of three or four bluetongue virus (BTV) genes respectively. When Spodoptera frugiperda cells were infected with the recombinant virus (AcBT-3/2/7/5) expressing the four major structural genes of BTV, double-capsid, virus-like particles consisting of VP2, VP3, VP5 and VP7 of BTV were assembled.