ATAC and SAGA co-activator complexes utilize co-translational assembly, but their cellular localization properties and functions are distinct.
ATAC and SAGA co-activator complexes utilize co-translational assembly, but their cellular localization properties and functions are distinct.
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DOI：
10.1016/j.celrep.2023.113099
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发表时间：
2023-09-26
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
为了了解多亚基复合物的功能，揭示指导其组装的精确机制至关重要。新生蛋白可以在翻译过程中找到并结合它们的相互作用伙伴，从而导致共翻译组装。在这里，我们证明了ATAC （ADA-two-A-containing）和SAGA （Spt-Ada-Gcn5-acetyltransferase）这两种含有赖氨酸乙酰转移酶的转录共激活因子复合物的核心模块在哺乳动物细胞的细胞质中进行共翻译组装。此外，含有其所有模块的SAGA复合体在细胞质中形成并使非组蛋白乙酰化。相比之下，在哺乳动物细胞的细胞质中无法检测到ATAC复合物亚基。然而，含有两个功能模块的内源性ATAC复合物在细胞核中形成并发挥作用。因此，两种相关的共激活因子ATAC和SAGA通过共翻译途径组装，但它们的亚细胞定位、细胞质丰度和功能是不同的。Yayli等人发现人类ATAC （ADA-two-A-containing）和SAGA （spt - ada - gcn5 -乙酰转移酶）转录共激活因子复合物模块在细胞质中共翻译组装。他们描述了完全组装的SAGA具有细胞质乙酰化功能。相比之下，ATAC共激活物复合物不能在细胞质中检测到，只能在细胞核中检测到。
To understand the function of multisubunit complexes, it is of key importance to uncover the precise mechanisms that guide their assembly. Nascent proteins can find and bind their interaction partners during their translation, leading to co-translational assembly. Here, we demonstrate that the core modules of ATAC (ADA-two-A-containing) and SAGA (Spt-Ada-Gcn5-acetyltransferase), two lysine acetyl transferase-containing transcription co-activator complexes, assemble co-translationally in the cytoplasm of mammalian cells. In addition, a SAGA complex containing all of its modules forms in the cytoplasm and acetylates non-histone proteins. In contrast, ATAC complex subunits cannot be detected in the cytoplasm of mammalian cells. However, an endogenous ATAC complex containing two functional modules forms and functions in the nucleus. Thus, the two related co-activators, ATAC and SAGA, assemble using co-translational pathways, but their subcellular localization, cytoplasmic abundance, and functions are distinct. Yayli et al. find that modules of human ATAC (ADA-two-A-containing) and SAGA (Spt-Ada-Gcn5-acetyltransferase) transcriptional co-activator complexes assemble co-translationally in the cytoplasm. They describe that fully assembled SAGA has cytoplasmic acetylation functions. In contrast, the ATAC co-activator complex cannot be detected in the cytoplasm, only in the nucleus.
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影响因子：
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通讯作者：
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影响因子：
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