Inhibition of fibrotic changes in infrapatellar fat pad alleviates persistent pain and articular cartilage degeneration in monoiodoacetic acid-induced rat arthritis model
Inhibition of fibrotic changes in infrapatellar fat pad alleviates persistent pain and articular cartilage degeneration in monoiodoacetic acid-induced rat arthritis model
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DOI：
10.1016/j.joca.2020.12.014
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发表时间：
2021-02-26
影响因子：
7
通讯作者：
Koga, H.
中科院分区：
文献类型：
--
作者：
An, J-S;Tsuji, K.;Koga, H.
目的：我们报道了大鼠急性关节炎症后髌下脂肪垫(IFP)的纤维化改变与持续性疼痛密切相关。在本研究中，为了观察抗纤治疗对持续性疼痛的影响，我们在急性关节炎症后的恢复期使用了C型利钠肽(CNP)。设计：使用32只雄性Wistar大鼠。关节腔内注射单碘乙酸(MIA)诱导IFP纤维化和持续性疼痛。在同一膝关节急性炎症期后注射CNP。结果：关节腔内注射CNP可抑制急性炎症后IFP的纤维化改变。失能试验表明，大鼠膝关节内注射MIA后，同侧肢体重量百分比迅速下降。在赋形剂组，这种下降持续到第28天，这表明疼痛持续发生在急性炎症之后(第0天/第28天，EST DIF-8.15，CI-10.78-5.53，线性混合效应模型)。而CNP组术后第14天(0天/14天，-0.51天，-2.62-1.59天)疼痛减轻。此外，我们在第14天观察到CNP组关节软骨退变程度显著改善(OARSI评分：VOARSI评分16.14+/-4.37vs CNP 6.87+/-3.44，P<；0.01；Wilcoxon秩和检验)。结论：IFP的纤维化改变可能在持续性疼痛和关节软骨退变中发挥重要作用。(C)2021年国际骨关节炎研究会。爱思唯尔有限公司出版。保留所有权利。
Objective: We have reported that fibrotic changes in infrapatellar fat pad (IFP) after acute joint inflammation are closely associated with persistent pain in rats. In this study, to examine the effects of anti-fibrotic treatment on persistent pain, we used C-type natriuretic peptides (CNP) at the recovery phase after acute joint inflammation.Design: Thirty-two male Wistar rats were used in this study. Monoiodoacetic acid (MIA) was injected intra-articularly to induce IFP fibrosis and persistent pain. CNP was injected after acute inflammatory phase in the same knee joint. Time-course pain-avoidance behavior tests and histological analyses were performed to examine the effects of CNP.Results: Histological evaluations indicated that intra-articular injection of CNP inhibited fibrotic changes in IFP after acute inflammation. Incapacitance tests indicated that MIA injection into rat knee joint quickly decreased the percent weight on ipsilateral limb. In the vehicle group, the decrease was maintained up to day 28, suggesting that pain persistence occurred after acute inflammation (Day 0/Day 28, Est Dif -8.15, CI -10.78--5.53, Linear mixed-effect model). In contrast, the pain was alleviated in the CNP group after day 14 (Day0/Day 14, -0.51, -2.62-1.59). In addition, we observed significant improvement in the degree of articular cartilage degeneration at day 14 in the CNP group (OARSI score: vehicle 16.14 +/- 4.37 vs CNP 6.87 +/- 3.44, P < 0.01; Wilcoxon rank sum test).Conclusion: Fibrotic changes in IFP may play important roles in both persistent pain and articular cartilage degeneration. (C) 2021 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.