The local integration preference of the Tf1 retrotransposon in Schizosaccharomyces pombe
The local integration preference of the Tf1 retrotransposon in Schizosaccharomyces pombe
复制标题
Tf1反转录转座子在粟酒裂殖酵母中的局部整合偏好
DOI：
10.1016/j.virol.2021.10.008
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发表时间：
2022-01-02
期刊：
影响因子：
3.7
通讯作者：
Guo，Yabin
中科院分区：
文献类型：
--
作者：
Cui，Yujin;Guo，Yabin
Transposons are mobile DNAs that can move to different locations in host genomes. The integration site selection of transposons is critical for both themselves and host cells. Studies on the integration of retrotransposons and retroviruses have focused more on the global preference than on the local preference. The local preferences of retrotransposons are usually weak and of large diversity. Here, we analyzed hundreds of thousands of independent integration events of the Tf1 retrotransposon inSchizosaccharomyces pombe. The consensus sequence at the Tf1 integration sites shows a palindromic pattern, which can be divided into four sections, each of them contains one or more CGnTA units with a period of 10 base pairs, indicating interaction with subunits of the integrase oligomer in the pre-integration complex. Moreover, the analysis on the nucleosome occupancy flanking Tf1 target sites shows that Tf1 integration favors regions with one entire nucleosome depletion.
