Serum OX40 ligand levels in asthmatic children: A potential biomarker of severity and persistence
Serum OX40 ligand levels in asthmatic children: A potential biomarker of severity and persistence
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DOI：
10.2500/aap.2011.32.3447
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发表时间：
2011-07-01
影响因子：
2.8
通讯作者：
Elbrhami, Emhemed Mossbah
中科院分区：
文献类型：
--
作者：
Ezzat, Mohamed Hesham Mohamed;Imam, Safaa Shafik;Elbrhami, Emhemed Mossbah
共刺激分子OX 40及其配体OX 40 L介导哮喘动物模型中过敏性气道炎症的关键方面，包括嗜酸性粒细胞气道炎症、气道高反应性和辅助性T细胞2型（Th 2）极化。然而，这些分子参与Th 2主导的过敏原诱导的儿童哮喘仍不清楚。因此，我们试图检查OX 40 L在儿童哮喘中的表达，包括疾病活动性和发作严重程度。采用ELISA法检测50例哮喘患儿血清OX 40 L浓度，并与40例健康儿童作对照。哮喘儿童急性发作期间血清OX 40 L水平的中位数和平均值（SD）（1487和1560 [543] pg/mL）显著高于发作之间的儿童（731和689 [321] pg/mL）和对照组（193和157 [60.3] pg/mL）。急性重度哮喘急性发作儿童的OX 40 L值高于轻度或中度哮喘急性发作儿童。在稳定期，重度持续性哮喘患者的水平显著高于中度或轻度持续性哮喘患者。急性发作期间的OX 40 L水平与缓解期间的相应值之间可能存在正相关性。血清OX 40 L水平与最大呼气流速呈负相关，与嗜酸性粒细胞绝对计数呈正相关。OX 40 L的上调可能在儿童特应性哮喘的发生发展中起重要作用，并与哮喘的严重程度相关。OX 40 L可能是监测过敏性炎症的一个有用的生物标志物。OX 40 L是治疗这些疾病的最有前途的免疫干预靶点之一。（Allergy Asthma Proc 32：313-318，2011; doi：10.2500/aap.2011.32.3447）
The costimulatory molecule OX40 and its ligand, OX40L, mediate key aspects of allergic airway inflammation in animal models of asthma, including eosinophilic airway inflammation, airway hyperresponsiveness, and T-helper type 2 (Th2) polarization. However, involvement of these molecules in Th2-dominated allergen-induced childhood asthma remains unclear. Therefore, we sought to examine OX40L expression in pediatric asthma across disease activity and attack severity. Serum OX40L concentrations were measured by ELISA in 50 children with atopic asthma (during and in between acute attacks), and in 40 healthy children serving as controls. The median and mean (SD) serum OX40L levels (1487 and 1560 [543] pg/mL) were significantly higher in asthmatic children during acute attacks in comparison with children in between attacks (731 and 689 [321] pg/mL) and in comparison with controls (193 and 157 [60.3] pg/mL). OX40L values were higher among children who presented with acute severe asthma exacerbations than in children with mild or moderate asthma exacerbations. During stability, patients with severe persistent asthma had significantly higher levels when compared with patients with moderate or mild persistent asthma. A positive correlation could be elicited between OX40L levels during exacerbations and the corresponding values during remission. Serum OX40L levels correlated negatively with peak expiratory flow rate and positively with absolute eosinophil count. Up-regulation of OX40L may play a critical role in development of childhood atopic asthma and is in favor of asthma severity. OX40L may represent a useful biomarker of monitoring allergic inflammation. OX40L is one of the most promising targets of immune intervention for treatment of these diseases. (Allergy Asthma Proc 32:313-318, 2011; doi: 10.2500/aap.2011.32.3447)