Novel device for application of continuous mechanical tensile strain to mammalian cells.
Novel device for application of continuous mechanical tensile strain to mammalian cells.
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DOI：
10.1242/bio.023671
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发表时间：
2017-04-15
期刊：
影响因子：
2.4
通讯作者：
Nakamura Y
中科院分区：
文献类型：
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作者：
Wada S;Kanzaki H;Narimiya T;Nakamura Y
在正畸牙齿移动过程中，牙周膜（PDL）暴露于持续的机械应变。然而，许多研究人员施加循环拉伸应变，而不是连续拉伸应变，在体外PDL细胞，因为一直没有足够的设备，以施加连续拉伸应变培养的细胞。在这项研究中，我们设计了一种新的装置，旨在施加连续的拉伸应变培养细胞。对人永生化牙周膜细胞系（HPL细胞）施加持续拉伸应变，用免疫组织化学方法观察HPL细胞的细胞骨架结构。炎症和成骨标志物的表达也通过实时逆转录聚合酶链反应进行了检查。Western blot检测成骨蛋白骨桥蛋白（OPN）的表达。HPL细胞的肌动蛋白丝在连续拉伸应变下呈均匀排列。持续张应变可增加HPL细胞IL-1β、IL-6、考克斯-2、TNF-α等炎性基因和RUNX 2、OPN等成骨基因的表达。HPL细胞中OPN蛋白的表达也明显增加。这些结果表明，我们的新的简单的装置是有用的，用于探索连续拉伸应变施加到细胞的反应。总结：器械的持续拉伸应变改变了细胞形态，增加了炎症和成骨基因的表达。这些影响类似于正畸牙齿移动过程中的PDL。
During orthodontic tooth movement, the periodontal ligament (PDL) is exposed to continuous mechanical strain. However, many researchers have applied cyclic tensile strain, not continuous tensile strain, to PDL cells in vitro because there has been no adequate device to apply continuous tensile strain to cultured cells. In this study, we contrived a novel device designed to apply continuous tensile strain to cells in culture. The continuous tensile strain was applied to human immortalized periodontal ligament cell line (HPL cells) and the cytoskeletal structures of HPL cells were examined by immunohistochemistry. The expression of both inflammatory and osteogenic markers was also examined by real-time reverse transcription polymerase chain reaction. The osteogenic protein, Osteopontin (OPN), was also detected by western blot analysis. The actin filaments of HPL cells showed uniform arrangement under continuous tensile strain. The continuous tensile strain increased the expression of inflammatory genes such as IL-1β, IL-6, COX-2 and TNF-α, and osteogenic genes such as RUNX2 and OPN in HPL cells. It also elevated the expression of OPN protein in HPL cells. These results suggest that our new simple device is useful for exploring the responses to continuous tensile strain applied to the cells. Summary: Continuous tensile strain from the device changed the cell morphology and increased the expression of inflammatory and osteogenic gene. These effects were similar to those in the PDL during orthodontic tooth movement.