Development of a micro dual beam fluorometric detector specific for microchip analysis of benzo[a]pyrene and benzo[k]fluoranthene in diesel exhaust particulate samples.
Development of a micro dual beam fluorometric detector specific for microchip analysis of benzo[a]pyrene and benzo[k]fluoranthene in diesel exhaust particulate samples.
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DOI：
10.1039/b504440e
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发表时间：
2005-08
期刊：
影响因子：
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通讯作者：
H. Masaki;H. Susaki;T. Korenaga
中科院分区：
文献类型：
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作者：
H. Masaki;H. Susaki;T. Korenaga
我们开发了一种检测苯并[a]芘（B[a]P）和苯并[k]荧光蒽（B[k]F）的装置和一些系统。该装置采用发射波长为370nm的紫外发光二极管和发射波长为395nm的紫色激光二极管作为激发光源。该检测方法基于以下观察：B[a]P和B[k]F均发出特征荧光，其强度比22种其他多环芳烃（PAHs）的荧光强度高10倍。B[a]P和B[k]F的荧光激发和发射波长分别为370 nm和429 nm， 395 nm和429 nm。此外，我们通过使用24种多环芳烃混合物确定的两条校准曲线计算了它们在柴油排气颗粒中的浓度。该微芯片装置对B[a]P和B[k]F的检出限分别为5.58 μ L（-1）和6.03 μ L（-1）。应用该方法对三种柴油机尾气颗粒物中的B[a]P和B[k]F进行了分析，结果与液相色谱法吻合较好。
We developed a device and some systems for detecting benzo[a]pyrene (B[a]P) and benzo[k]fluoranthene (B[k]F). The device uses a UV light-emitting diode that emits light with a wavelength of 370 nm and a violet laser diode that emits light with a wavelength of 395 nm as excitation light sources. The detection method is based on the following observation: characteristic fluorescence is emitted from both B[a]P and B[k]F, with intensities up to 10 times greater than those from 22 other polycyclic aromatic hydrocarbons (PAHs). The excitation and emission wavelengths for fluorescence from B[a]P and B[k]F are 370 and 429 nm, and 395 and 429 nm, respectively. Further, we calculated their concentrations in diesel exhaust particles by means of two calibration curves determined using a mixture of 24 PAHs. The detection limits of the proposed device with a microchip are 5.58 microg L(-1) for B[a]P and 6.03 microg L(-1) for B[k]F. We applied this method to analyze B[a]P or B[k]F contained in three types of diesel exhaust particles, and the results agreed well with those obtained by liquid chromatography.