EPISODIC SECRETION OF ACTH IN RATS
EPISODIC SECRETION OF ACTH IN RATS
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DOI：
10.1016/0196-9781(86)90216-0
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发表时间：
1986-03-01
期刊：
影响因子：
3
通讯作者：
BARKSDALE, CM
中科院分区：
文献类型：
--
作者：
CARNES, M;BROWNFIELD, MS;BARKSDALE, CM
虽然垂体促肾上腺皮质激素(ACTH)分泌的昼夜节律已经有了很好的特征，但对超常节律的研究较少。为了研究ACTH分泌的间歇性，自由、未麻醉的大鼠通过颈静脉插管持续放血，从1100小时(n=6)或1730小时(n=4)开始，每隔一分钟采集血液长达75分钟。在一天中的两个时间段都可以观察到零星的低幅度微脉冲。此外，在晚上还观察到了罕见的“超级脉冲”。对脉搏参数的分析表明，早晚之间脉搏幅度有显著差异(p<；0.001)，但脉搏频率或峰间间隔没有差异。与其他间歇性分泌激素一样，脉冲识别阈值和采样间隔也会影响观察到的脉冲参数。
While the circadian rhythm of pituitary adrenocorticotropin (ACTH) secretion has been well characterized, the ultradian rhythm has been less thoroughly investigated. To study the episodic nature of ACTH secretion, unrestrained, unanesthetized rats were bled continuously through indwelling jugular venous cannulae and blood sampled for up to 75 minutes at one-minute intervals beginning at 1100 hr (n = 6) or 1730 hr (n = 4). Sporadic low-amplitude micropulses were observed at both times of day. In addition, infrequent "superpulses" were observed in the evening. Analysis of pulse parameters revealed a significant (p < 0.001) difference in pulse amplitude but no difference in pulse frequency or interpeak interval between morning and evening. As with other episodically secreted hormones, the threshold for pulse identification and the sampling interval were found to influence the observed pulse parameters.