Light microscopy: an ongoing contemporary revolution
Light microscopy: an ongoing contemporary revolution
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DOI：
10.1080/00107514.2015.1026557
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发表时间：
2015-04-03
影响因子：
2
通讯作者：
Sandoghdar, Vahid
中科院分区：
文献类型：
--
作者：
Weisenburger, Siegfried;Sandoghdar, Vahid
光学显微镜是最古老的科学仪器之一，至今仍在前沿研究中使用。恩斯特·阿贝 (Ernst Abbe) 在 19 世纪提出的显微镜分辨率极限让几代科学家相信，对单个分子进行光学研究和解析亚波长结构是不可行的。 2014 年因超分辨率荧光显微镜而获得的诺贝尔奖标志着人们明确认识到必须重新审视旧的信念。在本文中，我们对光学显微镜的各种最新发展进行了批判性概述。除了流行的超分辨率荧光方法之外，我们还讨论了各种其他技术和成像对比度的前景，并考虑了未来的一些基本和实际挑战。
The optical microscope is one of the oldest scientific instruments that is still used in forefront research. Ernst Abbe's nineteenth century formulation of the resolution limit in microscopy let generations of scientists believe that optical studies of individual molecules and resolving subwavelength structures were not feasible. The Nobel Prize in 2014 for super-resolution fluorescence microscopy marks a clear recognition that the old beliefs have to be revisited. In this article, we present a critical overview of various recent developments in optical microscopy. In addition to the popular super-resolution fluorescence methods, we discuss the prospects of various other techniques and imaging contrasts and consider some of the fundamental and practical challenges that lie ahead.