GANGLIOSIDE GM2 AS A HUMAN-TUMOR ANTIGEN (OFA-I-1)
GANGLIOSIDE GM2 AS A HUMAN-TUMOR ANTIGEN (OFA-I-1)
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DOI：
10.1073/pnas.80.17.5392
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
IRIE, RF
中科院分区：
文献类型：
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作者：
TAI, T;PAULSON, JC;IRIE, RF
由[人类]在体外产生的一种单特异性抗体。eb病毒转化淋巴母细胞样细胞系可识别人肿瘤和胎儿脑上的膜抗原（OFA-I-1）。确定了OFA-I-1的化学性质。采用氯仿/甲醇/水4:8:3 （vol/vol）萃取ofa - i -1阳性黑色素瘤细胞系M14富抗原废培养基的糖脂部分，DEAE-Sephadex分离成中性糖脂和神经节苷部分，碱基处理，Bio-sil A柱洗脱。当用免疫粘附抑制试验检测单特异性抗OFA-I-1时，发现OFA-I-1抗原仅存在于神经节苷脂部分。抗原M14神经节苷脂的TLC和序列糖苷酶切结果表明抗原为神经节苷脂GM2， GalNAc.beta。1 .fwdarw。4 (NeuAc.alpha。2 .fwdarw。3) Gal.beta。1 .fwdarw。4、相关.fwdarw。Cer。通过对各种真实神经节苷和中性糖脂的检测，进一步证实了OFA-I-1的抗原性。只有GM2表现出阳性反应。
A monospecific antibody produced in vitro by a [human] .beta. lymphoblastoid cell line transformed with Epstein-Barr virus recognizes a membrane antigen (OFA-I-1) on human tumors and fetal brain. The chemical nature of OFA-I-1 is identified. The glycolipid fraction of antigen-rich spent medium of an OFA-I-1-positive melanoma cell line, M14, was extracted by chloroform/methanol/water, 4:8:3 (vol/vol), and was separated into fractions of neutral glycolipids and gangliosides by DEAE-Sephadex followed by base treatment and Bio-sil A column elution. OFA-I-1 antigens were found exclusively in the ganglioside fraction when assayed with monospecific anti-OFA-I-1 by an immune adherence inhibition test. The results obtained from TLC of the antigenic M14 ganglioside and sequential glycosidase digestion suggested that the antigen was a ganglioside GM2, GalNAc.beta.1 .fwdarw. 4 (NeuAc.alpha.2 .fwdarw. 3)Gal.beta.1 .fwdarw. 4Glc .fwdarw. Cer. The results were further supported by testing various authentic gangliosides and neutral glycolipids for OFA-I-1 antigenicity. Only GM2 showed positive reactivity.