Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin.
Midgut transcriptomal response of the rice leaffolder, Cnaphalocrocis medinalis (Guenée) to Cry1C toxin.
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水稻纵卷叶螟 (Guenée) 对 Cry1C 毒素的中肠转录反应
DOI：
10.1371/journal.pone.0191686
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Lu Z
中科院分区：
文献类型：
--
作者：
Yang Y;Xu H;Lu Y;Wang C;Lu Z
稻纵卷叶螟（Cnaphalocrocis medinalis（Guenée））是水稻的重要害虫之一。在C.稻纵卷叶螟防治和Bt水稻的培育是控制这种昆虫的策略。然而，昆虫对Bt蛋白的耐受性或抗性是Bt蛋白应用的主要威胁。为了研究C. medinalis转录组在防御Cry1C毒素中的作用，高通量RNA测序在C.用和不用Cry1C毒素处理的medinalis幼虫。在C.中肠比较分析确定了两种处理之间的6，966个不同表达的单基因（DEG）。GO分析表明这些基因参与蛋白质水解和胞外区。其中，羧酸酯酶、谷胱甘肽S-转移酶和P450在处理的C.中肠此外，胰蛋白酶，糜蛋白酶，羧肽酶在DEG中鉴定，其中大多数上调。此外，在Cry1C处理的C.中肠根据通路分析，抗原加工和呈递通路和慢性髓系白血病通路在C.用Cry1C毒素处理的medinalis。提示丝氨酸蛋白酶、解毒酶和ABC转运蛋白、抗原加工和呈递途径以及慢性粒细胞白血病途径可能参与了C.对Cry1C毒素的反应本研究为Bt Cry蛋白对C.为研究重要农业害虫麦长管蚜的耐性或抗性提供了潜在线索。对Cry1C毒素的反应
Cnaphalocrocis medinalis (Guenée) is one of the important insect pests in rice field. Bt agents were recommended in the C. medinalis control and Bt rice is bred as a tactic to control this insect. However, the tolerance or resistance of insect to Bt protein is a main threat to the application of Bt protein. In order to investigate the response of C. medinalis transcriptome in defending a Cry1C toxin, high-through RNA-sequencing was carried in the C. medinalis larvae treated with and without Cry1C toxin. A total of 35,586 high-quality unigenes was annotated in the transcriptome of C. medinalis midgut. The comparative analysis identified 6,966 differently expressed unigenes (DEGs) between the two treatments. GO analysis showed that these genes involved in proteolysis and extracellular region. Among these DEGs, carboxylesterase, glutathione S-transferase and P450 were differently expressed in the treated C. medinalis midgut. Furthermore, trypsin, chymotrypsin, and carboxypeptidase were identified in DEGs, and most of them up-regulated. In addition, thirteen ABC transporters were downregulated and three upregulated in Cry1C-treated C. medinalis midgut. Based on the pathway analysis, antigen processing and presentation pathway, and chronic myeloid leukemia pathway were significant in C. medinalis treated with Cry1C toxin. These results indicated that serine protease, detoxification enzymes and ABC transporter, antigen processing and presentation pathway, and chronic myeloid leukemia pathway may involved in the response of C. medinalis to Cry1C toxin. This study provides a transcriptomal foundation for the identification and functional characterization of genes involved in the toxicity of Bt Cry protein against C. medinalis, and provides potential clues to the studies on the tolerance or resistance of an agriculturally important insect pest C. medinalis to Cry1C toxin.
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影响因子：
3.4
作者：
Brunet, Jean-Frederic;Vachon, Vincent;Laprade, Raynald
通讯作者：
Laprade, Raynald
影响因子：
4.5
作者：
Gahan LJ;Pauchet Y;Vogel H;Heckel DG
通讯作者：
Heckel DG
影响因子：
3.4
作者：
Aurade, Ravindra M.;Jayalakshmi, Senigala K.;Sreeramulu, Kuruba
通讯作者：
Sreeramulu, Kuruba
影响因子：
29.7
作者：
Blum JS;Wearsch PA;Cresswell P
通讯作者：
Cresswell P
影响因子：
3.8
作者：
Corrêa S;Binato R;Du Rocher B;Castelo-Branco MT;Pizzatti L;Abdelhay E
通讯作者：
Abdelhay E