Direct measurement of intracellular pressure.
Direct measurement of intracellular pressure.
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DOI：
10.1002/0471143030.cb1209s63
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发表时间：
2014-06-03
影响因子：
--
通讯作者：
Koo, Hyun
中科院分区：
文献类型：
--
作者：
Petrie, Ryan J;Koo, Hyun
本单元描述了一种直接测量贴壁、迁移细胞的细胞内压力的方法。该方法基于零伺服方法，其中将微电极引入细胞中以直接测量细胞质的物理压力。我们还描述了微电极的初始校准，以及该方法在三维 (3-D) 细胞外基质 (ECM) 内迁移的细胞的应用。
A method to directly measure the intracellular pressure of adherent, migrating cells is described in this unit. This approach is based on the servo-null method where a microelectrode is introduced into the cell to directly measure the physical pressure of the cytoplasm. We also describe the initial calibration of the microelectrode, as well as the application of the method to cells migrating inside three-dimensional (3-D) extracellular matrix (ECM).