Ectopic expression of TrPI, a Taihangia rupestris (Rosaceae) PI ortholog, causes modifications of vegetative architecture in Arabidopsis
Ectopic expression of TrPI, a Taihangia rupestris (Rosaceae) PI ortholog, causes modifications of vegetative architecture in Arabidopsis
复制标题
TrPI（一种蔷薇科）PI 直系同源物 TrPI 的异位表达导致拟南芥营养结构的改变
DOI：
10.1016/j.jplph.2010.06.028
复制
发表时间：
2010-01-01
影响因子：
4.3
通讯作者：
Meng, Zheng
中科院分区：
文献类型：
--
作者：
Lue, Shanhua;Fan, Yinglun;Meng, Zheng
在真双子叶模式植物中，B功能基因编码一对配偶体MADS结构域蛋白，拟南芥中的APETALA 3（AP 3）和PISTILLATA（PI），金鱼草中的DEFICIENS（DEF）和GLOBOSA（GLO）。这些蛋白质，必须形成异二聚体的功能，需要指定花瓣和雄蕊的身份在花发育过程中。本文报道了真双子叶植物太行花（Taihangiarupestris PISTILLATA）的一个PI/GLO-like基因TrPI的克隆和特性分析。DNA凝胶印迹分析表明，TrPI基因在T.岩藻基因组定量RT-PCR和原位杂交分析表明，TrPI在营养和生殖器官中的转录水平不同。TrPI在拟南芥中的异位表达引起营养植物结构的严重修改，包括莲座叶和茎生叶排列在非螺旋状叶序中，以及在基部、中部或顶部产生两个或三个分支的扁平的主花序茎。此外，我们表明，TrPI基因是能够拯救PI-1突变表型。酵母双杂交试验表明TrPI形成同源二聚体。这些结果表明，TrPI除了作为一个花器官识别基因在T.这表明TrPI蛋白具有将其与充分研究的直系同源物PI和GLO区分开的生化特征。(C)2010年Elsevier GmbH。All rights reserved.
In eudicotyledonous model plants, the B-function genes encode a pair of partner MADS-domain proteins, APETALA3 (AP3) and PISTILLATA (PI) in Arabidopsis and DEFICIENS (DEF) and GLOBOSA (GLO) in Antirrhinum. These proteins, which must form heterodimers to function, are required to specify petal and stamen identity during flower development. Here, we report cloning and characterization of TrPI (Taihangia rupestris PISTILLATA), a PI/GLO-like gene from the core eudicot species Taihangia rupestris (Rosaceae). DNA gel blot analysis showed that TrPI is a single copy gene in the T. rupestris genome. Quantitative RT-PCR and in situ hybridization analyses revealed that TrPI is transcribed in both the vegetative and reproductive organs at different levels. Ectopic expression of TrPI in Arabidopsis caused severe modifications in vegetative plant architecture, including rosette leaves and cauline leaves arranged in a non-spiral phyllotaxy, and a flattened primary inflorescence stem that produced two or three offshoots at the base, middle or top. Moreover, we show that the TrPI gene is capable of rescuing pi-1 mutant phenotypes. Yeast two-hybrid assays showed that TrPI forms homodimers. Taken together, these results show that TrPI might function in regulating plant architecture in addition to its function as a floral organ identity gene in T. rupestris, suggesting that the TrPI protein has biochemical features that distinguish it from the well-studied orthologs, PI and GLO. (C) 2010 Elsevier GmbH. All rights reserved.