H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44
H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44
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DOI：
10.1128/jvi.02065-07
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发表时间：
2008-02-01
影响因子：
5.4
通讯作者：
Porgador, Angel
中科院分区：
文献类型：
--
作者：
Ho, Joanna W.;Hershkovitz, Oren;Porgador, Angel
抗病毒免疫防御涉及自然杀伤(NK)细胞。我们先前的研究表明，NK激活受体NKp44参与了NK细胞对H1型流感病毒株的功能识别。在本研究中，我们研究了NKp44与H5N1流感病毒分离株血凝素的相互作用。在这里，我们证明了重组NKp44识别表达H5的细胞，并与可溶性H5血凝素特异性地相互作用。H5假型慢病毒颗粒与表达NKp44的NK细胞结合。在与表达H5、伪型慢病毒颗粒或膜相关H5的靶细胞相互作用后，NK细胞显示出NKp44介导的诱导活性。这些发现表明，NKp44-H5相互作用可诱导功能性NK激活。
Antiviral immune defenses involve natural killer (NK) cells. We previously showed that the NK-activating receptor NKp44 is involved in the functional recognition of H1-type influenza virus strains by NK cells. In the present study, we investigated the interaction of NKp44 and the hemagglutinin of a primary influenza virus H5N1 isolate. Here we show that recombinant NKp44 recognizes H5-expressing cells and specifically interacts with soluble H5 hemagglutinin. H5-pseudotyped lentiviral particles bind to NK cells expressing NKp44. Following interaction with target cells expressing H5, pseudotyped lentiviral particles, or membrane-associated H5, NK cells show NKp44-mediated induced activity. These findings indicate that NKp44-H5 interactions induce functional NK activation.