A YEAST ACTIN-RELATED PROTEIN HOMOLOGOUS TO THAT IN VERTEBRATE DYNACTIN COMPLEX IS IMPORTANT FOR SPINDLE ORIENTATION AND NUCLEAR MIGRATION
A YEAST ACTIN-RELATED PROTEIN HOMOLOGOUS TO THAT IN VERTEBRATE DYNACTIN COMPLEX IS IMPORTANT FOR SPINDLE ORIENTATION AND NUCLEAR MIGRATION
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DOI：
10.1016/0092-8674(94)90531-2
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发表时间：
1994-08-26
期刊：
影响因子：
64.5
通讯作者：
COOPER, JA
中科院分区：
文献类型：
--
作者：
MUHUA, L;KARPOVA, TS;COOPER, JA
纺锤体方向控制着有丝分裂过程中的核迁移和分离。在酵母中，动力蛋白和星形微管的缺陷导致异常纺锤体取向和核迁移。Dynactin复合物是体外动力蛋白介导的囊泡运动所必需的。动力肌动蛋白复合物的主要多肽是Arp 1家族中的肌动蛋白相关蛋白。我们在S.酿酒酵母Arp 1家族中的一个新的肌动蛋白相关基因-β-肌动蛋白。act 5无效突变体在纺锤体方向和核迁移方面有缺陷，Act 5 p的过表达也是如此。缺乏动力蛋白和Act 5 p的双突变体的表型与单突变体的表型相似。因此，动力肌动蛋白复合物与动力蛋白处于相同的通路，并且可能是动力蛋白在体内发挥作用所必需的。
Spindle orientation controls nuclear migration and segregation during mitosis. In yeast, defects in dynein and astral microtubules lead to abnormal spindle orientation and nuclear migration. Dynactin complex is necessary for dynein-mediated vesicle motility in vitro. The major polypeptide of dynactin complex is an actin-related protein in the family Arp1. We have identified in S. cerevisiae a novel actin-related gene, ACTS, in the Arp1 family. An act5 null mutant has defects in spindle orientation and nuclear migration, as does overexpression of Act5p. The phenotype of a double mutant lacking dynein and Act5p is similar to that of single mutants. Therefore, dynactin complex is in the same pathway as dynein and may be necessary for the action of dynein in vivo.