Pan-Genomic Analysis ofClostridium botulinumGroup II (Non-ProteolyticC. botulinum) Associated with Foodborne Botulism and Isolated from the Environment
Pan-Genomic Analysis ofClostridium botulinumGroup II (Non-ProteolyticC. botulinum) Associated with Foodborne Botulism and Isolated from the Environment
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DOI：
10.3390/toxins12050306
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发表时间：
2020-05-01
期刊：
影响因子：
4.2
通讯作者：
Peck, Michael W.
中科院分区：
文献类型：
--
作者：
Brunt, Jason;van Vliet, Arnoud H. M.;Peck, Michael W.
由肉毒梭菌II型形成的神经毒素是食源性肉毒中毒的主要原因，肉毒中毒是一种致命的中毒。本研究的目的是了解C.肉毒杆菌II组菌株及其神经毒素基因。对208个高度分化的C进行了比较基因组研究。肉毒杆菌群II菌株（80年来从16个国家分离的180个新测序菌株，28个序列来自Genbank）。菌株具有单一的B、E或F型神经毒素基因，或者是没有神经毒素基因的密切相关菌株。鉴定了具有独特氨基酸序列的肉毒杆菌神经毒素亚型变体（包括新变体）。核心基因组单核苷酸多态性（SNP）分析确定了两个主要的谱系-一个与E型菌株，第二个占主导地位的亚型B4菌株与亚型F6菌株。这项研究揭示了人口结构/多样性的新细节，并建立了全基因组谱系，肉毒杆菌神经毒素亚型变异，与食源性肉毒中毒，流行病学和地理来源之间的关系。此外，基因组序列代表了研究界的宝贵资源（例如，了解C.肉毒杆菌及其神经毒素基因，剖析C.肉毒杆菌II组生物学）。这可能有助于改善风险评估和预防食源性肉毒杆菌中毒。
The neurotoxin formed byClostridium botulinumGroup II is a major cause of foodborne botulism, a deadly intoxication. This study aims to understand the genetic diversity and spread ofC. botulinumGroup II strains and their neurotoxin genes. A comparative genomic study has been conducted with 208 highly diverseC. botulinumGroup II strains (180 newly sequenced strains isolated from 16 countries over 80 years, 28 sequences from Genbank). Strains possessed a single type B, E, or F neurotoxin gene or were closely related strains with no neurotoxin gene. Botulinum neurotoxin subtype variants (including novel variants) with a unique amino acid sequence were identified. Core genome single-nucleotide polymorphism (SNP) analysis identified two major lineages-one with type E strains, and the second dominated by subtype B4 strains with subtype F6 strains. This study revealed novel details of population structure/diversity and established relationships between whole-genome lineage, botulinum neurotoxin subtype variant, association with foodborne botulism, epidemiology, and geographical source. Additionally, the genome sequences represent a valuable resource for the research community (e.g., understanding evolution ofC. botulinumand its neurotoxin genes, dissecting key aspects ofC. botulinumGroup II biology). This may contribute to improved risk assessments and the prevention of foodborne botulism.