Visualization of hormone binding proteins in vivo based on Mn-doped CdTe Qps
Visualization of hormone binding proteins in vivo based on Mn-doped CdTe Qps
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基于 Mn 掺杂 CdTe Qps 的体内激素结合蛋白可视化
DOI：
10.1016/j.saa.2014.04.066
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发表时间：
2014-10-15
影响因子：
4.4
通讯作者：
Wu, Jian zhong
中科院分区：
文献类型：
--
作者：
Liu, Fang fei;Yu, Ying;Wu, Jian zhong
氨酰肼（Daminozide，B 9）是一种植物生长抑制剂，对植物的生长发育具有重要的调节作用。在植物组织中定位和定量B 9结合蛋白将有助于研究B 9信号转导背后的机制。本研究采用高温水热法合成了红色荧光锰掺杂碲化镉量子点（CdTeMn量子点）。由于CdTeMn量子点在610 nm处具有最大荧光发射峰，因此其荧光性质比CdTe量子点更稳定。将B 9与CdTeMn量子点偶联，合成了B 9-CdTeMn探针。该探针的荧光强度是CdTeMn量子点的两倍;其荧光稳定性在不同的环境条件下也是上级的。该探针保留了B 9的生物活性，不受植物中存在的绿色荧光蛋白的干扰。因此，我们用这种探针标记B 9结合蛋白选择性绿豆幼苗根组织切片。这些蛋白主要在皮层和表皮薄壁组织的细胞膜表面上观察到。(C)2014爱思唯尔有限公司版权所有。
Daminozide (B9) is a growth inhibitor with important regulatory roles in plant growth and development. Locating and quantifying B9-binding proteins in plant tissues will assist in investigating the mechanism behind the signal transduction of B9. In this study, red fluorescent Mn-doped CdTe quantum dots (CdTeMn QDs) were synthesized by a high-temperature hydrothermal process. Since CdTeMn QDs possess a maximum fluorescence emission peak at 610 nm, their fluorescence properties are more stable than those of CdTe QDs. A B9-CdTeMn probe was synthesized by coupling B9 with CdTeMn QDs. The fluorescence intensity of the probe is double that of CdTeMn QDs; its fluorescence stability is also superior under different ambient conditions. The probe retains the biological activity of B9 and is unaffected by interference from the green fluorescent protein present in plants. Therefore, we used this probe to label B9-binding proteins selectively in root tissue sections of mung bean seedlings. These proteins were observed predominantly on the surfaces of the cell membranes of the cortex and epidermal parenchyma. (C) 2014 Elsevier B.V. All rights reserved.