EFFECTS OF EXPERIMENTAL SUPPRESSION OF ACTIVE (REM) SLEEP DURING EARLY DEVELOPMENT UPON ADULT BRAIN AND BEHAVIOR IN THE RAT
EFFECTS OF EXPERIMENTAL SUPPRESSION OF ACTIVE (REM) SLEEP DURING EARLY DEVELOPMENT UPON ADULT BRAIN AND BEHAVIOR IN THE RAT
复制标题
DOI：
10.1016/0165-3806(83)90184-0
复制
发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
BOER, GJ
中科院分区：
文献类型：
--
作者：
MIRMIRAN, M;SCHOLTENS, J;BOER, GJ
为了验证主动睡眠（AS）对中枢神经系统正常发育的重要性这一假设，对出生后1个月的雄性Wistar大鼠幼鼠采用3种不同的剥夺方法。每天注射氯丙咪嗪从8-21天的年龄降低了高水平的AS小于成人值在整个实验期间的大部分。从8-21天的生命可乐定管理诱导几乎完全抑制AS。工具剥夺，使用钟摆，方法，导致一个显着的（但不太严重）AS减少在出生后2-4周的年龄。成年期的旷场行为测试显示，所有3个实验组中的Ambadium水平均高于正常水平。在氯丙咪嗪和可乐定处理的动物中，由于坐骑和射精水平低，雄性性反应不足。无论是被动回避学习，也没有黑暗偏好测试显示实验组和对照组大鼠之间的任何差异。睡眠观察结果表明，有一个异常高的发病率大肌阵挛痉挛在AS在氯丙咪嗪和可乐定治疗的大鼠。随后测量的区域脑重量显示显着减少，在大脑皮层和延髓，与各自的对照组相比，在氯丙咪嗪和可乐定治疗的大鼠。此外，受影响的大脑区域的DNA和蛋白质测定显示皮质和髓质中的比例减少。在早期发育过程中干扰AS本身或特定单胺能递质系统的正常功能，可在以后的生活中产生长期的行为以及脑形态和生化异常。
In order to test the hypothesis that active sleep (AS) is important for the normal development of the CNS, 3 different deprivation methods were applied to male Wistar rat pups during the 1st month of life. Daily injection of clomipramine from 8-21 days of age reduced the high level of AS to less than the adult value throughout most of the experimental period. Administration of clonidine from 8-21 days of life induced an almost total suppression of AS. Instrumental deprivation, using the pendulum, method, led to a significant (but less severe) AS reduction during 2-4 wk of postnatal age. Open-field behavior testing in adulthood revealed a higher than normal level of ambulation in all 3 experimental groups. Masculine sexual responses were deficient, due to a low level of both mounts and ejaculations, in both clomipramine- and clonidine-treated animals. Neither passive avoidance learning nor dark preference tests revealed any differences between the experimental and control rats. Sleep observations showed that there was an abnormally high incidence of large myoclonic jerks during AS in both clomipramine- and clonidine-treated rats. Subsequent measurement of regional brain weights showed a significant reduction in the cerebral cortex and medulla oblongata, as compared with the respective control groups, in both the clomipramine- and the clonidine-treated rats. In addition, DNA and protein determination in the affected brain areas showed a proportional reduction in the cortex and in the medulla. Interference with normal functioning either of AS per se or of specific monoaminergic transmitter systems during early development can produce long-lasting behavioral as well as brain morphological and biochemical abnormalities in later life.