Study on the interaction and antioxidant activity of theophylline and theobromine with SOD by spectra and calculation.
Study on the interaction and antioxidant activity of theophylline and theobromine with SOD by spectra and calculation.
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DOI：
10.1016/j.saa.2019.03.001
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发表时间：
2019-05
期刊：
影响因子：
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通讯作者：
Fufang Wu;Ruirui Liu;Xiaobao Shen;Huajie Xu;Liangquan Sheng
中科院分区：
文献类型：
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作者：
Fufang Wu;Ruirui Liu;Xiaobao Shen;Huajie Xu;Liangquan Sheng
茶碱（TP）和可可碱（TB）是黄嘌呤的甲基衍生物。研究了茶多酚和茶多酚的抗氧化作用以及茶多酚和茶多酚对铜锌超氧化物歧化酶（SOD）活性和抗氧化作用的影响。细胞内MDA含量测定结果表明，TP（14.49 μmol/g）和TB（14.25 μmol/g）均具有抗氧化活性，与SOD（13.77 μmol/g）的抗氧化作用相近。随着TP-SOD和TB-SOD的形成，抗氧化能力可以叠加。用紫外光谱、荧光光谱和分子对接等方法研究了TP/TB与SOD的相互作用。结果表明，TP/TB与SOD形成1：1的复合物，其稳定性主要由氢键和货车范德华力共同作用。分析还表明，SOD的微环境和结构发生了变化。以上结果表明，TP-SOD和TB-SOD复合物的形成可以保持各自的抗氧化作用，而不影响SOD的活性。
Theophylline (TP) and theobromine (TB) are the methyl derivatives of xanthine. The antioxidation of TP and TB as well as the effect of the antioxidation and activity of copper‑zinc superoxide dismutase (SOD) with TP and TB were investigated. The contents of MDA in cells showed that both TP (14.49 μmol/g) and TB (14.25 μmol/g) are active in oxidation resistance and closed to the antioxidant effect of SOD (13.77 μmol/g). With the formation of TP-SOD and TB-SOD, the antioxidant ability can be superimposed. The interactions between TP/TB and SOD were studied by ultraviolet spectrum, fluorescence spectrum and molecular docking. The results showed that the complex of TP/TB and SOD with 1:1 component was stabilized by hydrogen bonding and van der Waals forces. The analysis also indicated that the microenvironment and structure of SOD were changed. All of the results indicate that the complex formation of TP-SOD and TB-SOD can maintain their respective antioxidant effects without changes in the activity of SOD.