ELISA FOR DETECTION OF GROUP-COMMON AND VIRUS-SPECIFIC ANTIBODIES IN HUMAN AND SIMIAN SERA INDUCED BY HERPES-SIMPLEX AND RELATED SIMIAN VIRUSES
ELISA FOR DETECTION OF GROUP-COMMON AND VIRUS-SPECIFIC ANTIBODIES IN HUMAN AND SIMIAN SERA INDUCED BY HERPES-SIMPLEX AND RELATED SIMIAN VIRUSES
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DOI：
10.1016/0166-0934(86)90040-6
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发表时间：
1986-09-01
影响因子：
3.1
通讯作者：
LIPPER, SL
中科院分区：
文献类型：
--
作者：
KATZ, D;HILLIARD, JK;LIPPER, SL
建立了一种快速（3.5 h）酶联免疫吸附试验（ELISA），用于检测血清中单纯疱疹病毒1型（HSV-1）和两种抗原相关的猴病毒，猴因子8（SA 8）和猴疱疹病毒（B病毒）的抗体。去污剂溶解的感染细胞和类似处理的对照模拟感染细胞的粗制品用作包被微孔板中的威尔斯孔的抗原。使用生物素化蛋白A和抗生物素蛋白结合的碱性磷酸酶检测不同物种（人、猴和兔）血清中的抗体。三个原型检测描述了三个程度的特异性。病毒抗原的共同或特异性测定可在简单的竞争试验中使用与包被威尔斯孔的抗原制剂相似的抗原制剂进行测定。特异性检测允许对人和猴疱疹病毒的抗体进行快速鉴别血清学诊断。
A rapid (3.5 h) enzyme-linked immunosorbent assay (ELISA) was developed for the detection of serum antibodies to herpes simplex virus type 1 (HSV-1) and to two antigenically related monkey viruses, simian agent 8 (SA8) and Herpesvirus simiae (B virus). Crude preparations of detergent solubilized infected cells and similarly treated control mock-infected cells served as antigens for coating wells in microplates. Biotinylated protein A and avidin-conjugated alkaline phosphatase were used to detect antibodies in sera from different species (humans, monkeys and rabbits). Three prototype assays are described with three degrees of specificity. Common or specific determinations on the viral antigens could be assayed in simple competition tests using similar antigen preparations to those coating the wells. The specific assays permitted rapid differential serodiagnosis of antibodies to human and simian herpesviruses.