COMPARISON OF VIRULENCE MARKERS OF PERITONEAL AND FALLOPIAN-TUBE ISOLATES WITH ENDOCERVICAL NEISSERIA-GONORRHOEAE ISOLATES FROM WOMEN WITH ACUTE SALPINGITIS
COMPARISON OF VIRULENCE MARKERS OF PERITONEAL AND FALLOPIAN-TUBE ISOLATES WITH ENDOCERVICAL NEISSERIA-GONORRHOEAE ISOLATES FROM WOMEN WITH ACUTE SALPINGITIS
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DOI：
10.1128/iai.27.3.882-888.1980
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发表时间：
1980-01-01
影响因子：
3.1
通讯作者：
SWEET, RL
中科院分区：
文献类型：
--
作者：
DRAPER, DL;JAMES, JF;SWEET, RL
N.引起急性输卵管炎的淋病菌株可能从子宫颈沿生殖道上行。以前的研究利用从宫颈管培养物中获得的分离株，尽管尚不清楚分离株是否真正代表输卵管中存在的微生物。N.将来自宫颈管培养物的淋病分离物与输卵管或腹膜盲囊分离物或来自腹腔镜检查获得的两个部位的分离物进行比较。研究了潜在的毒力标记，包括菌落表型、营养型、抗菌剂敏感性、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上的蛋白质模式和对正常人血清的敏感性。在7株宫颈分离株中，6株的主要外膜蛋白的电泳图谱和分子量与相应的腹膜分离株相同。菌株型是相同的，包括原养型、脯氨酸需要型和脯氨酸和脯氨酸需要型菌株。作为一个群体的分离株似乎对正常妇女的混合血清的杀菌作用非常敏感。菌落表型不同的网站之间，输卵管盲囊分离株主要是透明的表型和纤毛。宫颈分离株是等量不透明和透明表型或主要不透明表型的混合物。通过这些标记，患者N。淋病宫颈分离株与其从输卵管分离的分离株似乎相同，除了菌落表型的差异或改变。
N. gonorrhoeae strains which cause acute salpingitis probably ascend the genital tract from the cervix. Previous studies utilized isolates obtained from endocervical canal cultures, although it was not known if the isolates truly represented the organisms present in the fallopian tubes. N. gonorrhoeae isolates from endocervical canal cultures were compared with fallopian tube or peritoneal cul-de-sac isolates or isolates from both sites obtained at laparoscopy. Potential virulence markers were studied, including colony phenotype, auxotype, antimicrobial agent susceptibility, protein patterns on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and susceptibility to normal human serum. Of 7 cervical isolates, 6 had the same antibiograms and MW for major outer membrane proteins as those of the corresponding peritoneal isolates. Auxotypes were the same and included prototrophic, proline-requiring and proline-and-arginine-requiring isolates. The isolates as a group appeared very susceptible to the bactericidal action of pooled serum from normal women. Colony phenotypes varied between sites; the fallopian tubes cul-de-sac isolates were predominantly of transparent phenotype and piliated. The cervical isolates were mixtures of equal quantities of opaque and transparent phenotypes or predominantly opaque phenotype. By these markers, patients'' N. gonorrhoeae cervical isolates appeared the same as their isolates from fallopian tubes except for a difference or shift in colony phenotype.