Genome-wide association study and biological pathway analysis of the Eimeria maxima response in broilers
Genome-wide association study and biological pathway analysis of the Eimeria maxima response in broilers
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DOI：
10.1186/s12711-015-0170-0
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发表时间：
2015-11-25
影响因子：
4.1
通讯作者：
Bed'Hom, Bertrand
中科院分区：
文献类型：
--
作者：
Hamzic, Edin;Buitenhuis, Bart;Bed'Hom, Bertrand
背景：球虫病是家禽业中最常见和最昂贵的疾病，由艾美耳属的原生动物引起。目前对球虫病的控制是以使用抗球虫药物和接种疫苗为基础的，这两种方法分别面临着抗药性和开发有效疫苗的高成本等严重障碍。因此，目前的控制程序必须扩大与互补的方法，如使用遗传学来提高宿主对艾美耳球虫感染的反应。最近，我们用大肠杆菌对Cobb 500肉鸡进行了大规模的攻毒试验。最大值，我们研究了动物对挑战的反应的变异性。作为这项挑战研究的后续工作，我们进行了全基因组关联研究（GWAS），以确定基因组区域的变异性的测量性状的反应，在肉鸡极大艾美耳球虫。此外，我们进行了GWAS后功能分析，以增加我们对巨型艾美耳球虫挑战的潜在反应的生物学理解。结果：我们总共鉴定了22个具有q值的单核苷酸多态性（SNP）
Background: Coccidiosis is the most common and costly disease in the poultry industry and is caused by protozoans of the Eimeria genus. The current control of coccidiosis, based on the use of anticoccidial drugs and vaccination, faces serious obstacles such as drug resistance and the high costs for the development of efficient vaccines, respectively. Therefore, the current control programs must be expanded with complementary approaches such as the use of genetics to improve the host response to Eimeria infections. Recently, we have performed a large-scale challenge study on Cobb500 broilers using E. maxima for which we investigated variability among animals in response to the challenge. As a follow-up to this challenge study, we performed a genome-wide association study (GWAS) to identify genomic regions underlying variability of the measured traits in the response to Eimeria maxima in broilers. Furthermore, we conducted a post-GWAS functional analysis to increase our biological understanding of the underlying response to Eimeria maxima challenge.Results: In total, we identified 22 single nucleotide polymorphisms (SNPs) with q value