A VERY STRONG ENHANCER IS LOCATED UPSTREAM OF AN IMMEDIATE EARLY GENE OF HUMAN CYTOMEGALO-VIRUS
A VERY STRONG ENHANCER IS LOCATED UPSTREAM OF AN IMMEDIATE EARLY GENE OF HUMAN CYTOMEGALO-VIRUS
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DOI：
10.1016/s0092-8674(85)80025-8
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
SCHAFFNER, W
中科院分区：
文献类型：
--
作者：
BOSHART, M;WEBER, F;SCHAFFNER, W
人巨细胞病毒（HCMV）是疱疹病毒组中一种普遍存在的严重病原体，在HCMV的基因组DNA中发现了一种强转录增强子（235内切酶）。无增强子的SV 40 DNA与随机片段化的HCMV DNA共转染产生了2个SV 40-HCMV重组病毒，其并入了HCMV DNA的重叠片段以取代缺失的SV 40增强子。在HCMV内，这些增强子序列位于主要立即早期基因的转录起始位点的上游，在核苷酸-118和-524之间。HCMV增强子含有多种重复序列基序，其缺失研究表明，该增强子的不同子集可以取代SV 40增强子。HCMV增强子似乎对细胞类型或种属没有偏好性，其活性比SV 40增强子高几倍。它是迄今为止分析的最强增强子，这一特性使其成为真核表达载体的有用组分。
A strong transcription enhancer was identified in the genomic DNA (235 kilobase) of human cytomegalovirus (HCMV), a ubiquitous and severe pathogen of the herpesvirus group. Cotransfection of enhancerless SV40 DNA with randomly fragmented HCMV DNA yielded 2 SV40-HCMV recombinant viruses that had incorporated overlapping segments of HCMV DNA to substitute for the missing SV40 enhancer. Within HCMV, these enhancer sequences are located upstream of the transcription initiation site of the major immediate-early gene, between nucleotides -118 and -524. Deletion studies with the HCMV enhancer, which harbors a variety of repeated sequence motifs, show that different subsets of this enhancer can substitute for the SV40 enhancer. The HCMV enhancer, which seems to have little cell type or species preference, is severalfold more active than the SV40 enhancer. It is the strongest enhancer analyzed so far, a property that makes it a useful component of eukaryotic expression vectors.