Assessing nuclear and mitochondrial DNA sequence variation within steinernema (rhabditida: steinernematidae).
Assessing nuclear and mitochondrial DNA sequence variation within steinernema (rhabditida: steinernematidae).
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评估斯氏线虫（rhabditida：斯氏线虫科）内的核和线粒体 DNA 序列变异。
作者：
Allen L. Szalanski;D. Taylor;P. Mullin
DNA序列分析被用来表征核核糖体DNA ITS 1区和部分的COII和16 S rDNA基因的线粒体基因组斯氏线虫昆虫病原线虫。七种斯氏线虫核ITS 1核苷酸差异分析5个种间的差异在6 ~ 22%之间，5个种间的差异在12 ~ 20%之间。3个S. Feltiae strains.核和线粒体DNA序列的系统发育分析证实了现有的形态关系的几个斯氏线虫物种。rDNA ITS 1和mtDNA序列对斯氏线虫分类群之间的亲缘关系的分析都是有用的。
DNA sequence analysis was used to characterize the nuclear ribosomal DNA ITS1 region and a portion of the COII and 16S rDNA genes of the mitochondrial genome from Steinernema entomopathogenic nematodes. Nuclear ITS1 nucleotide divergence among seven Steinernema spp. ranged from 6 to 22%, and mtDNA divergence among five species ranged from 12 to 20%. No intraspecific variation was observed among three S. feltiae strains. Phylogenetic analysis of both nuclear and mitochondrial DNA sequences confirms the existing morphological relationships of several Steinernema species. Both the rDNA ITS1 and mtDNA sequences were useful for resolving relationships among Steinernema taxa.