Cancer stem-like cells with hybrid epithelial/mesenchymal phenotype leading the collective invasion
Cancer stem-like cells with hybrid epithelial/mesenchymal phenotype leading the collective invasion
复制标题
具有混合上皮/间质表型的癌症干细胞样细胞引领集体侵袭
DOI：
10.1111/cas.14285
复制
发表时间：
2020-01-17
期刊：
影响因子：
5.7
通讯作者：
Yang, Gen
中科院分区：
文献类型：
--
作者：
Quan, Qianghua;Wang, Xudong;Yang, Gen
癌细胞的集体侵袭是循环肿瘤细胞（CTC）簇形成的关键过程，并极大地促进了转移。癌症干细胞样细胞（CSC）具有明显的迁移转移优势。为了验证CSC在集体侵袭中的作用，我们进行了3D测定来研究癌细胞球体的集体侵袭。结果表明，CSC可以显着促进集体和单细胞侵袭。进一步的研究表明，CSC更喜欢迁移到外部，并领导集体入侵。更有趣的是，集体侵袭中约60%的领导CSC共表达上皮和间充质基因，而在单一侵袭性CSC中仅4%共表达，表明具有混合上皮/间充质表型的CSC在癌细胞集体侵袭中起关键作用。
Collective invasion of cancer cells is the key process of circulating tumor cell (CTC) cluster formation, and greatly contributes to metastasis. Cancer stem-like cells (CSC) have a distinct advantage of motility for metastatic dissemination. To verify the role of CSC in the collective invasion, we performed 3D assays to investigate the collective invasion from cancer cell spheroids. The results demonstrated that CSC can significantly promote both collective and single-cell invasion. Further study showed that CSC prefer to move outside and lead the collective invasion. More interestingly, approximately 60% of the leader CSC in collective invasion co-expressed both epithelial and mesenchymal genes, while only 4% co-expressed in single invasive CSC, indicating that CSC with hybrid epithelial/mesenchymal phenotype play a key role in cancer cell collective invasion.