Secondary structure model for mouse beta Maj globin mRNA derived from enzymatic digestion data, comparative sequence and computer analysis.
Secondary structure model for mouse beta Maj globin mRNA derived from enzymatic digestion data, comparative sequence and computer analysis.
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小鼠 beta Maj 珠蛋白 mRNA 的二级结构模型源自酶消化数据、比较序列和计算机分析。
DOI：
10.1093/nar/14.14.5827
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发表时间：
1986
影响因子：
14.9
通讯作者：
Maizel,J
中科院分区：
文献类型：
--
作者：
Lockard,RE;Currey,K;Browner,M;Lawrence,C;Maizel,J
基于酶解数据、比较序列和计算机分析，建立了小鼠βMajglobin信使RNA二级结构模型。以5′- 32p末端标记的β珠蛋白mRNA为底物，用s1和t1核酸酶测定单链区域，用v1核糖核酸酶测定双链区域。将获得的约75%的分子结构数据引入计算机算法，该算法预测与酶解数据一致的最小自由能二级结构。计算机生成的结构中也存在两个突出的碱基配对区域，这些区域是由系统发育分析独立得出的，为模型提供了支持。该模型的一个有趣特征是存在远程碱基配对相互作用，这种相互作用允许β珠蛋白mRNA折叠回自身，从而使5 ‘和3 ’非编码区更接近。这一特征与其他实验室的数据一致，表明哺乳动物珠蛋白mrna中的5 ‘和3 ’结构域存在相互作用。
A model for the secondary structure of mouse βMajglobin messenger RNA is presented based on enzymatic digestion data, comparative sequence and computer analysis. Using 5′-32P-end-labeled β globin mRNA as a substrate, single-stranded regions were determined with S1and T1nucleases and double-stranded regions with V1ribonuclease from cobra venom. The structure data obtained for ca. 75% of the molecule was introduced into a computer algorithm which predicts secondary structures of minimum free energy consistent with the enzymatic data. Two prominent base paired regions independently derived by phylogenetic analysis were also present in the computer generated structure lending support for the model. An interesting feature of the model is the presence of long-range base pairing interactions which permit the β globin mRNA to fold back on itself, thereby bringing the 5′- and 3′-noncoding regions within close proximity. This feature is consistent with data from other laboratories suggesting an interaction of the 5′- and 3′-domains in the mammalian globin mRNAs.