C-FOS EXPRESSION DURING ELECTROCUPUNCTURE ANALGESIA IN RATS - AN IMMUNOHISTOCHEMICAL STUDY
C-FOS EXPRESSION DURING ELECTROCUPUNCTURE ANALGESIA IN RATS - AN IMMUNOHISTOCHEMICAL STUDY
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DOI：
10.3727/036012992816357738
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发表时间：
1992-07-01
影响因子：
0.3
通讯作者：
XU, SF
中科院分区：
文献类型：
--
作者：
DAI, JL;ZHU, YH;XU, SF
虽然电针镇痛的中枢机制已被研究，但由于传统技术难以追踪神经元通路，对中枢神经系统（CNS）神经元参与电针镇痛的系统研究还没有很好地进行。最近发展的c-fos表达检测免疫组化方法与Ab-1抗血清可能用于此目的，作为一个有用的标记，在中枢神经系统的神经活动。本研究以甩尾潜伏期（TFL）作为清醒大鼠痛阈的指标。单侧电针“钻三里”、“环条”后，TFL明显延长。为探讨EAA可能涉及中枢神经系统的某些神经元群，我们观察了EAA伴发的c-fos蛋白在整个轴突中的表达，并在腰髓中发现了大量特异性的c-fos蛋白标记神经元（板层I和II），中缝大核，中缝背核，中央灰质，外侧缰核，内侧缰核，丘脑内侧核，下丘脑外侧核、乳头体上核、视上核、视前内侧核、杏仁核、斜角束核等，对照组大鼠上述区域均无明显c-fos表达。上述结果表明，电针对上述核团的功能激活是EAA作用的基础。
Although the central mechanisms of electroacupuncture analgesia (EAA) have been investigated, a systematic study for the involvement of neuronal populations of central nervous system (CNS) in EAA has not been well undertaken, largely due to the difficulty in tracing the neuronal pathways by traditional techniques. Recently developed c-fos expression examination by immunohistochemical method with Ab-1 antisera might be used for this purpose as a useful marker for neuronal activity in CNS. In this study, tail flick latency (TFL) was tested as an index of pain threshold in conscious rats. After unilateral electroacupuncture was applied at 'Zuan-san-li' and 'Huan-tiao', the TFL was significantly prolonged. To explore the possible involvement of certain neuronal groups of central nervous system in EAA, we examined the EAA accompanied c-fos expression throughout the neuraxis, and a lot of specific c-fos protein labelled neurons were found in lumbar spinal cord (laminae I and II), nucleus raphe magnus, nucleus raphe dorsalis, substantia grisea centralis, nucleus habenulae lateralis, nucleus habenulae medialis, nucleus medialis thalami, nucleus lateralis hypothalami, nucleus supramamillaris, nucleus supraopticus, nucleus arcuatus, nucleus preopticus medialis, nucleus amygdala, nucleus tractus diagonalis, etc. No obvious c-fos expression was shown in these areas on control rats. These results strongly suggested that the functional activation of above-mentioned nuclei by electroacupuncture was underlied in EAA action.