Xiaoyaosan-containing serum inhibits corticosterone-induced apoptosis in PC12 cells by downregulating [Ca2+]i overload
Xiaoyaosan-containing serum inhibits corticosterone-induced apoptosis in PC12 cells by downregulating [Ca2+]i overload
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DOI：
10.3969/j.issn.1673-5374.2010.18.006
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发表时间：
2010-09-30
影响因子：
6.1
通讯作者：
Tan, Hongmei
中科院分区：
文献类型：
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作者：
Li, Lin;Zhang, Xuanhong;Tan, Hongmei
海马神经元凋亡是海马神经元[Ca 2 +]浓度降低的重要因素，随着皮质酮浓度的增加，海马神经元[Ca 2 +]浓度迅速升高，导致海马神经元凋亡。逍遥散可能通过下调[Ca ~（2+）]来抑制细胞凋亡。（i）用含逍遥散的血清给药，测定了细胞凋亡、[Ca 2 +]（i）超负荷和线粒体膜电位的变化大剂量逍遥散含药血清与生物能量学灌胃给药剂量为2633 g/mL，不仅能抑制细胞形态学损伤和细胞凋亡，还能抑制[Ca ~（2+）]（i）皮质酮所致的线粒体膜电位和腺苷三磷酸水平的降低。依赖性抑制皮质酮诱导的细胞凋亡，可能通过抑制[Ca 2 +]（i）过载，并通过维持线粒体膜电位和三磷酸腺苷的产生来防止受损的线粒体生物能量学，为其抗抑郁作用提供了假定的机制
Apoptosis in hippocampal neurons is an important contributor to depression [Ca2+], of hippocampal neurons may raise rapidly with increased corticosterone concentrations, leading to apoptosis The anti-depressant effects of Xiaoyaosan (Free and Easy Wanderer's Powder) may inhibit apoptosis by down regulating the [Ca2+](i) overload PC12 cells were containing with corticosterone to simulate neuronal injury, dosed with Xiaoyaosan-containing serum, and the following parameters were measured apoptosis, [Ca2+](i) overload, and changes in mitochondrial membrane potential (Delta Psi m) and bioenergetics High-dose Xiaoyaosan-containing serum (the dose of intragastric administration was 2 633 g herb/mL) not only inhibited morphological injury and cell apoptosis, but also suppressed [Ca2+](i) overload induced by corticosterone Xiaoyaosan also blocked the decrease in mitochondrial membrane potential and adenosine triphosphate levels induced by corticosterone Xiaoyaosan dose-dependently suppresses corticosterone-induced apoptosis, potentially via inhibiting [Ca2+](i) overload, and prevents impaired mitochondrial bioenergetics by maintaining mitochondrial membrane potential and adenosine triphosphate production, providing a putative mechanism for its antidepressant effects