Studies on primary cultures of differentiated fetal liver cells.
Studies on primary cultures of differentiated fetal liver cells.
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DOI：
10.1083/jcb.52.3.559
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发表时间：
1972-03
期刊：
影响因子：
--
通讯作者：
Paul D
中科院分区：
文献类型：
--
作者：
Leffert HL;Paul D
描述了一种培养非生长或缓慢生长的分化的胎鼠肝细胞的方法。它涉及使用胶原酶作为消化剂和缺乏精氨酸的选择性培养基，精氨酸抑制非实质肝细胞的生长。有证据表明，存活的细胞（a）保留肝脏特异性尿素循环功能，通过其将鸟氨酸转化为精氨酸的能力进行测量，（B）在葡萄糖缺乏的培养基中合成DNA，（c）合成和分泌白蛋白。这种原代细胞培养响应部分肝切除大鼠血清，并可能是一个适当的测定系统的机制，调节肝再生的研究。
A method for culturing non- or slowly growing, differentiated fetal rat liver cells is described. It involves the use of collagenase as a digesting agent and of a selective medium deficient in arginine which suppresses the growth of nonparenchymal liver cells. Evidence is presented that surviving cells (a) retain liver-specific urea cycle functions measured by their capacity to transform ornithine into arginine, (b) synthesize DNA in glucose-deficient medium, and (c) synthesize and secrete albumin. This primary cell culture responds to partially hepatectomized rat serum and may be an appropriate assay system for the study of mechanisms which regulate liver regeneration.